Anti-COX5B Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P10606

Core Product Specifications and Parameters

Biological Background: COX5B Function and Localization

• COX5B (cytochrome c oxidase subunit 5B, mitochondrial) is a nuclear-encoded subunit of the terminal enzyme of the mitochondrial respiratory chain, complex IV (cytochrome c oxidase).
• It is integral to the electron transport chain, facilitating the reduction of molecular oxygen to water and coupling this to proton translocation across the inner mitochondrial membrane to generate the electrochemical gradient for ATP synthesis.
• The COX5B gene encodes a 129-amino acid preproprotein that is processed to a mature ~14 kDa subunit (residues 32–129), which is part of the catalytic core of complex IV.
• Subcellularly, COX5B localizes to the mitochondrial inner membrane.
• The protein contains a zinc-binding site and is subject to post-translational modifications including acetylation, as identified by proteomic studies.
• COX5B is widely expressed and plays a housekeeping role in oxidative phosphorylation across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature human COX5B peptide (amino acids 32–129), ensuring reactivity with the processed subunit.
• For Western blot, a prominent band at ~14 kDa should be observed in mitochondrial-enriched or whole-cell lysates from human, mouse, and rat tissues.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), verify specificity using positive control tissues rich in mitochondria (e.g., heart, skeletal muscle, kidney) and consider antigen-retrieval protocols.
• For immunoprecipitation (IP) and ELISA, pre-validation in the target sample system and assessment of cross-reactivity with complex IV assembly intermediates is recommended.
• Polyclonal antibodies may exhibit lot-to-lot variability; include appropriate controls and validate performance for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, core labs, and research institutes with streamlined access to a broad inventory of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-COX5B antibody supports mitochondrial biology and oxidative phosphorylation research, and we can assist with sourcing rare targets, alternative clones, and customized production to accelerate your assay development and clinical translation.