Anti-COX5B Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P10606

Core Product Specifications and Parameters

Biological Background: COX5B Function and Localization

• COX5B, also known as cytochrome c oxidase subunit 5B or COXVB, is a nuclear-encoded component of complex IV (cytochrome c oxidase) in the mitochondrial electron transport chain.
• It catalyzes the terminal reduction of molecular oxygen to water, coupling electron transfer to proton pumping across the inner mitochondrial membrane to drive ATP synthesis.
• The protein localizes specifically to the mitochondrial inner membrane, where it associates with other subunits of the cytochrome c oxidase holoenzyme.
• Together with complexes II and III, COX5B contributes to the respiratory chain that transfers electrons from NADH and succinate to oxygen, establishing an electrochemical gradient.
• The active site of cytochrome c oxidase includes heme A3 and copper B (CuB), with COX5B playing a structural role in maintaining the integrity and function of the binuclear center.
• Post-translational modifications include acetylation, and the protein binds a zinc ion (metal-binding), as identified by proteomics and direct protein sequencing.
• The mature protein (after cleavage of the mitochondrial transit peptide) has a molecular weight of approximately 14 kDa, matching the bulk of the mature subunit.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 29–129 of human COX5B, a region that corresponds to the mature protein after removal of the mitochondrial targeting sequence; this design enhances recognition of the endogenous processed form.
• For Western blotting (WB), consider using reducing conditions and mitochondrial-enriched protein extracts to maximize detection of the ~14 kDa band.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), COX5B staining typically exhibits a punctate mitochondrial pattern; validate with organelle markers to confirm localization.
• The antibody cross-reacts with mouse and rat COX5B; verify reactivity in the relevant species’ cell or tissue lysates, as sequence homology in the immunogen region may vary.
• For ELISA applications, optimize coating conditions and antibody dilutions using recombinant COX5B or mitochondrial fractions from human, mouse, or rat samples.
• As a monoclonal antibody, it is suitable for consistent lot-to-lot performance; nevertheless, each new application should be titrated and validated in the intended sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic developers and research laboratories, offering validated monoclonal antibodies such as this anti-COX5B clone alongside comprehensive mitochondrial research reagents. Our supply chain spans bulk antibody pairs, ancillary reagents, and rare-target sourcing—all backed by technical consultation from concept to clinic. Whether you need optimized immunoreagents for oxidative phosphorylation studies or scalable production support, CamelBio bridges the gap between discovery and diagnostic deployment.