Anti-COX5A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P20674

Core Product Specifications and Parameters

Biological Background: COX5A Function and Localization

• COX5A encodes cytochrome c oxidase subunit 5A, mitochondrial, also known as cytochrome c oxidase polypeptide Va.
• It is a critical component of complex IV (cytochrome c oxidase), the terminal enzyme of the mitochondrial electron transport chain.
• Complex IV catalyzes the reduction of molecular oxygen to water, using electrons from reduced cytochrome c, contributing to the proton gradient that drives ATP synthesis.
• The subunit is anchored in the mitochondrial inner membrane, with a transit peptide directing its import into the organelle.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation, suggesting regulatory roles in mitochondrial function.
• Mutations in COX5A are linked to primary mitochondrial disease, highlighting its essential role in cellular energy metabolism.
• The protein contains heme A and copper centers as cofactors, essential for electron transfer within complex IV.
• COX5A has a mature molecular weight of approximately 17 kDa, consistent with its detection by western blot.

Experimental Guidance and Technical Tips

• For western blot (WB), a predominant band near 17 kDa is expected in mitochondrial-rich samples; optimize loading controls and validate in relevant tissue or cell lines.
• When performing immunohistochemistry (IHC-P), consider heat-induced antigen retrieval using citrate or EDTA buffer and confirm staining specificity with appropriate controls.
• For immunofluorescence (IF/ICC), permeabilize cells to access the mitochondrial compartment; co-staining with a mitochondrial marker can help verify localization.
• In ELISA applications, this polyclonal antibody may be used as capture or detection reagent; assay conditions such as coating concentration and blocking should be individually optimized.
• The reported cross-reactivity with mouse and rat allows translational studies, but species-specific epitope recognition should be confirmed in your model system.
• As a polyclonal antibody, lot-to-lot consistency is not guaranteed; for quantitative or regulated assays, validate each new lot against previous performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of antibodies, recombinant proteins, and ancillary reagents for diagnostic manufacturers and research laboratories. In the field of mitochondrial biology and oxidative phosphorylation, CamelBio can supply validated antibody pairs, monoclonal and polyclonal antibodies targeting complex IV subunits like COX5A, and bulk reagents to support assay development. With a commitment to quality and reliability, CamelBio bridges your sourcing needs from concept to clinic, including access to rare targets and customized solutions.