Anti-COX5A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P20674

Core Product Specifications and Parameters

Biological Background: COX5A Function and Localization

• COX5A, also known as cytochrome c oxidase polypeptide Va, is a nuclear-encoded subunit of the mitochondrial respiratory chain complex IV (cytochrome c oxidase).
• It is an essential component of the last enzyme in the electron transport chain, which drives oxidative phosphorylation by reducing molecular oxygen to water.
• The protein contains a transit peptide and is localized to the mitochondrial inner membrane.
• COX5A participates in electron transfer from cytochrome c to the active binuclear center, contributing to proton pumping and ATP synthesis.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity.
• Mutations in COX5A are associated with primary mitochondrial disease.
• It has been identified by proteomics and direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-150), which includes the mature protein; consider epitope accessibility in native versus denaturing conditions.
• For WB, a band around 16 kDa is expected; validate with appropriate controls including COX5A knockout lysates.
• For IHC-P, antigen retrieval may be required; optimization of antibody dilution and detection system is recommended.
• Cross-reactivity with mouse and rat suggests utility in common model organisms; verify specificity in the relevant tissue or cell type.
• ELISA performance may depend on antigen coating; use the recombinant immunogen or purified protein as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This COX5A polyclonal antibody is relevant for mitochondrial biology and oxidative phosphorylation research, supporting assay development in metabolic disease areas. Contact our team to streamline procurement of rare-target reagents and custom formulations for your diagnostic workflow.