Anti-COX5A Monoclonal Antibody (KD Validated) for WB, IHC-P, IF, ELISA - P20674

Core Product Specifications and Parameters

Biological Background: COX5A Function and Localization

• COX5A encodes cytochrome c oxidase subunit 5A, a nuclear-encoded subunit of mitochondrial complex IV, the terminal enzyme of the electron transport chain.
• The protein is essential for the reduction of molecular oxygen to water, a process coupled to proton pumping for ATP synthesis.
• It is localized to the mitochondrial inner membrane, contributing to the structural integrity and function of the cytochrome c oxidase complex.
• COX5A contains heme and metal-binding cofactors necessary for electron transfer.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, which may regulate activity or assembly.
• Mutations in COX5A are associated with primary mitochondrial disease, highlighting its critical role in cellular respiration.
• The protein has a transit peptide for mitochondrial import and its structure has been determined, supporting molecular studies.

Experimental Guidance and Technical Tips

• The antibody was generated using a recombinant protein immunogen, suggesting recognition of native COX5A; however, validation in the intended sample type is recommended.
• For WB, a band near 17 kDa is expected; mitochondrial fraction enrichment may improve signal.
• In IHC-P and IF, mitochondrial staining patterns should be observed; consider antigen retrieval methods appropriate for mitochondrial epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as an integrated supplier of IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents for global diagnostic developers. For mitochondrial research targets like COX5A, we can provide optimized monoclonal antibodies and support bulk procurement needs, facilitating your assay development from concept to clinical application.