Anti-COX2/PTGS2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q05769

Core Product Specifications and Parameters

Biological Background: COX2/PTGS2 Function and Localization

• Prostaglandin G/H synthase 2 (PTGS2, also known as COX-2, PES-2, PHS-2, TIS10, gripghs) is encoded by the Ptgs2 gene and serves as the inducible cyclooxygenase mediating prostanoid biosynthesis in inflammatory responses.
• PTGS2 catalyzes the bis-oxygenation and peroxidation of arachidonic acid to form prostaglandin G2 (PGG2) and subsequently PGH2, the pivotal intermediate for prostaglandins and thromboxanes (PubMed:12925531, PubMed:20463020, PubMed:20810665). Related references: PMID:12925531 PMID:20463020 PMID:20810665
• The enzyme accepts dihomo-γ-linoleate and eicosapentaenoate as substrates, generating series-1 and series-3 prostaglandins, respectively (By similarity).
• COX-2 engages in the biosynthesis of specialized pro-resolving mediators (SPMs) such as D-series resolvins from docosahexaenoate (DHA) and contributes to neuroinflammation-resolving lipoxin A4 production (PubMed:29662056). Related references: PMID:29662056
• Subcellularly, COX-2 resides in microsomal, endoplasmic reticulum, and nuclear membrane compartments.
• Post-translational modifications including acetylation, S-nitrosylation, and glycosylation modulate its function.
• It is prominently induced in wound-bed mesenchymal cells during colonic injury repair (at protein level).
• The predicted molecular mass of mouse COX-2 is ~69 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 101-210 of mouse COX-2 (NP_035328.2). When using this antibody, consider epitope accessibility and validate in mouse-derived samples.
• For Western blot (WB), a band near 69 kDa is anticipated; perform appropriate positive/negative controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), confirm localization patterns in macrophages or other cell types known to express COX-2.
• ELISA applications may be optimized by checkerboard titration to determine optimal antibody and antigen concentrations.
• Cross-reactivity is reported for mouse; verify specificity if analyzing samples from other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting researchers and manufacturers targeting the COX-2/PGE2 inflammatory pathway. We provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your diagnostic development from concept to clinic. Partner with us to accelerate your prostaglandin research and assay build.