Anti-COX2/PTGS2 Rabbit Monoclonal Antibody for WB - P35354

Core Product Specifications and Parameters

Biological Background: COX2/PTGS2 Function and Localization

• COX2/PTGS2, also known as cyclooxygenase-2, is a dual-function enzyme with cyclooxygenase and peroxidase activities that catalyzes the conversion of arachidonic acid to prostaglandin H2 (PGH2). Related references: PMID:16373578, PMID:22942274, PMID:26859324
• It plays a central role in the inflammatory response and is the key target of nonsteroidal anti-inflammatory drugs (NSAIDs). Related references: PMID:11939906, PMID:16373578, PMID:19540099
• Beyond inflammation, COX2 oxygenates other polyunsaturated fatty acids to generate specialized pro-resolving mediators (SPMs) such as resolvins, which aid in resolution of inflammation. Related references: PMID:12391014, PMID:21206090, PMID:26236990
• Subcellularly, the enzyme is anchored in the membrane of the endoplasmic reticulum, microsome, and both the inner and outer nuclear membranes.
• COX2 requires heme and iron as cofactors for its enzymatic activity, as indicated by UniProt keyword annotations.
• Post-translational modifications including acetylation, disulfide bond formation, and glycosylation are predicted based on sequence features.
• Alternative names include PHS-2, PGHS-2, and GRIPGHS, reflecting its prostaglandin synthase activity.
• Metabolizing 2-arachidonoyl glycerol, COX2 contributes to the biosynthesis of prostanoid glyceryl esters implicated in pain perception. Related references: PMID:22942274

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; therefore, the antibody recognizes a linear epitope, making it suitable for applications requiring denaturing conditions (e.g., WB).
• For Western blot, the predicted molecular weight is 69 kDa; consider using a molecular weight marker to confirm band identity.
• The antibody is validated for human, mouse, and rat samples; verify cross-reactivity in your model system if working with other species.
• Rabbit monoclonal antibodies provide consistent batch-to-batch performance and high specificity, advantageous for IHC-P and IP.
• Optimize fixation and antigen retrieval conditions for IHC-P as formaldehyde fixation can mask epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development. This anti-COX2/PTGS2 antibody supports research in inflammation and cancer, with CamelBio providing streamlined access to rare-target raw materials and technical consultation from concept to clinic.