Anti-COX2/PTGS2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P35354

Core Product Specifications and Parameters

Biological Background: COX2/PTGS2 Function and Localization

• COX2/PTGS2 (Prostaglandin G/H synthase 2) is an inducible dual-function enzyme that catalyzes the cyclooxygenation of arachidonic acid to prostaglandin G2 (PGG2) and its subsequent peroxidation to prostaglandin H2 (PGH2), the central precursor of all prostanoids. Related references: PMID:16373578 PMID:22942274 PMID:26859324
• Beyond arachidonate, COX2 oxygenates other polyunsaturated fatty acids such as dihomo-γ-linolenic acid (DGLA) and eicosapentaenoic acid (EPA) to produce alternative prostanoid series, contributing to diverse lipid mediator profiles. Related references: PMID:11939906 PMID:19540099
• In addition to classical prostanoid synthesis, COX2 participates in the generation of specialized pro-resolving mediators (SPMs) including D-series resolvins from docosahexaenoic acid (DHA) and E-series resolvins from EPA, playing a key role in the resolution of inflammation. Related references: PMID:12391014 PMID:21206090
• COX2 is predominantly localized to the membranes of the endoplasmic reticulum, microsomes, and both inner and outer nuclear membranes, positioning it at key intracellular sites for eicosanoid signaling.
• The enzyme requires heme and iron cofactors for its dual activities and is subject to post-translational modifications including acetylation, S-nitrosylation, and disulfide bond formation, which may regulate its function.
• COX2 metabolizes 2-arachidonoyl glycerol to prostanoid glyceryl esters, a process linked to pain perception, and can also oxygenate linoleic acid to hydroxyoctadecadienoates (HODEs). Related references: PMID:22942274

Experimental Guidance and Technical Tips

• The immunogen is designed against the C-terminal region (aa 505-604) of human COX2, an area with high sequence homology across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot, the predicted molecular weight is ~69 kDa; ensure appropriate gel percentage and transfer conditions. Validate with appropriate positive controls (e.g., COX2-induced cell lysates) and consider the inducible nature of the protein.
• In IHC-P, antigen retrieval may be necessary; optimize retrieval conditions for your specific tissue and fixation method. Including both positive (e.g., inflamed tissue) and negative (COX2-low) controls is recommended.
• For ELISA, confirm the peptide epitope recognition in your assay format, and consider using recombinant COX2 protein or peptide as a standard if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with consolidated access to high-quality IVD raw materials. This COX2/PTGS2 monoclonal antibody exemplifies our commitment to supporting inflammation and lipid mediator research, and we can further assist with complementary antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets in the prostanoid pathway.