Anti-COX2/PTGS2 Polyclonal Antibody for WB, IHC-P, ELISA - P35354

Core Product Specifications and Parameters

Biological Background: COX2/PTGS2 Function and Localization

• Prostaglandin G/H synthase 2 (COX-2) is a dual-function enzyme with cyclooxygenase and peroxidase activities that catalyze the conversion of arachidonic acid to prostaglandin H2 (PGH2), the precursor of prostaglandins and thromboxanes involved in inflammation (PubMed:16373578, PubMed:22942274, PubMed:26859324). Related references: PMID:16373578, PMID:22942274, PMID:26859324

• COX-2 also metabolizes other polyunsaturated fatty acids, including dihomo-γ-linolenate and eicosapentaenoate, to generate 1- and 3-series prostaglandins, and contributes to the biosynthesis of specialized pro-resolving mediators (resolvins) from omega-3 fatty acids (PubMed:11939906, PubMed:19540099, PubMed:12391014). Related references: PMID:11939906, PMID:19540099, PMID:12391014

• Subcellularly, COX-2 localizes primarily to the endoplasmic reticulum, microsomes, and nuclear membranes, positioning it to rapidly respond to stimuli.

• The enzyme is a glycoprotein with a heme cofactor and disulfide bonds, and its activity is modulated by post-translational modifications including acetylation and S-nitrosylation.

• PTGS2/COX-2 is a member of the prostaglandin G/H synthase family and is classified under the oxidoreductase, peroxidase, and dioxygenase enzyme classes.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 69 kDa is expected, though post-translational modifications such as glycosylation may cause slight shifts in electrophoretic mobility.
• In IHC-P, consider optimizing antigen retrieval conditions (e.g., heat-induced epitope retrieval in citrate buffer) to achieve specific staining.
• Use appropriate positive and negative controls to validate specificity in your sample system; cross-reactivity with mouse and rat has been reported.
• The immunogen spans amino acids 375–604, a region that may be conserved across species; verify performance if used in non-tested organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, including this COX2/PTGS2 polyclonal antibody for inflammation and prostaglandin research. Our sourcing network offers validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to support assay development. For rare or custom targets, CamelBio leverages a global supply chain to accelerate your project from concept to clinic.