Anti-COX1/PTGS1 Rabbit Monoclonal Antibody for WB, ELISA - P23219

Core Product Specifications and Parameters

Biological Background: COX1/PTGS1 Function and Localization

• COX1/PTGS1 is a dual cyclooxygenase and peroxidase enzyme that catalyzes the conversion of arachidonic acid to prostaglandin H2 (PGH2), the key precursor for all 2-series prostaglandins and thromboxanes. This reaction involves a bis-oxygenase activity that inserts two oxygen molecules, followed by reduction of the hydroperoxy intermediate. Related references: PMID:7947975
• The gene undergoes alternative splicing, producing isoforms that may have distinct expression or functional profiles.
• COX1 is constitutively expressed in many tissues, with critical roles in gastric epithelial cells for cytoprotective prostaglandin E2 (PGE2) synthesis, and in platelets for thromboxane A2 (TXA2) production, which promotes platelet aggregation and vasoconstriction.
• Beyond arachidonic acid, COX1 can metabolize linoleic acid to produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner.
• Subcellularly, COX1 localizes to the microsome and endoplasmic reticulum membranes, consistent with its role in lipid metabolism.
• The protein contains heme and iron cofactors, is glycosylated, and includes an EGF-like domain, all contributing to its enzymatic function and stability.
• COX1 is a key player in the inflammatory response and is often studied in the context of nonsteroidal anti-inflammatory drug (NSAID) mechanisms, which typically inhibit its cyclooxygenase activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 50–150 of human COX1/PTGS1. This N-terminal region may be suitable for detecting the full-length protein, but consider potential epitope masking or isoform-specific recognition.
• For Western blot, the predicted molecular weight is approximately 69 kDa. However, actual migration may vary due to glycosylation or other post-translational modifications; validate with appropriate positive controls.
• In ELISA applications, the antibody can be used as either a capture or detection reagent, depending on assay design. Pairing with another antibody or verifying performance in indirect ELISA formats is advisable.
• Cross-reactivity is indicated for human and mouse. When working with other species, perform a sequence alignment of the immunogen region to assess potential reactivity, and verify experimentally if needed.
• For functional studies, consider the antibody's ability to recognize the native conformation and whether it might interfere with enzyme activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing solutions for diagnostic manufacturers, labs, and research institutes, supporting projects from concept to clinic. This COX1/PTGS1 antibody fits into workflows investigating prostaglandin biosynthesis, inflammation, and platelet-related pathways. We offer a wide range of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing for rare targets—ensuring seamless integration into your assay development and manufacturing needs.