Anti-COX1/PTGS1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P23219

Core Product Specifications and Parameters

Biological Background: Prostaglandin G/H synthase 1 Function and Localization

• COX1 (Prostaglandin G/H synthase 1), encoded by PTGS1, is the constitutive cyclooxygenase isoform primarily responsible for prostaglandin production in the gastric mucosa and platelets.
• It catalyzes the conversion of arachidonic acid to prostaglandin H2 via sequential cyclooxygenase and peroxidase activities, initiating the biosynthesis of all 2-series prostaglandins and thromboxanes (PubMed:7947975). Related references: PMID:7947975
• The enzyme is an integral membrane protein of the endoplasmic reticulum and microsomal membranes, consistent with its role in eicosanoid biosynthesis.
• COX1 is a heme-containing glycoprotein with an EGF-like domain, disulfide bonds, and alternative splicing variants (UniProt keywords).
• In the stomach, COX1-derived prostaglandin E2 contributes to cytoprotection; in platelets, it generates thromboxane A2 to promote aggregation and vasoconstriction.
• It can also metabolize linoleic acid to specific hydroxyoctadecadienoic acids (HODEs) in a stereospecific manner (By similarity).

Experimental Guidance and Technical Tips

• The immunogen, an N-terminal fragment (amino acids 1-180), may enable detection of both full-length COX1 and potential cleavage products; validate specificity with positive and negative controls.
• For Western blot, consider the predicted molecular weight of ~69 kDa when selecting protein markers and transfer conditions.
• In immunohistochemistry, heat-induced epitope retrieval methods may improve signal; optimize for your specific tissue type and fixation protocol.
• The antibody's cross-reactivity with mouse and rat supports its use across species, but verify performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-COX1 polyclonal antibody is part of our comprehensive portfolio for inflammation and eicosanoid research. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and offer rare target raw-material sourcing to support your workflows.