Anti-COX15 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q7KZN9

Core Product Specifications and Parameters

Biological Background: COX15 Function and Localization

• COX15, also known as heme A synthase or cytochrome c oxidase assembly protein COX15 homolog, is a mitochondrial inner membrane protein critical for the final step of heme A biosynthesis.
• It catalyzes the conversion of heme O to heme A through two successive hydroxylations of the C8 methyl group, a reaction that requires matrix ferredoxin and ferredoxin reductase. Related references: PMID:12474143
• The mature enzyme is a multi-pass transmembrane protein located in the mitochondrial inner membrane, consistent with its role in oxidative phosphorylation.
• Expression is highest in tissues with elevated energy demands, such as muscle, heart, and brain, where cytochrome c oxidase activity is essential.
• Alternative splicing generates multiple transcript variants, potentially contributing to tissue-specific regulation or isoform diversity.
• Mutations in COX15 are associated with Leigh syndrome (MC4DN6), a severe mitochondrial disorder, and other primary mitochondrial diseases, highlighting its clinical relevance.
• The protein belongs to the COX15 family and contains iron-binding sites, reflecting its oxidoreductase activity and metal-dependent catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 150–250 of human COX15, an internal region. Consider using this antibody in Western blot under reducing conditions for optimal detection.
• For immunohistochemistry (IHC), antigen retrieval and mitochondrial membrane permeabilization may be necessary to expose the epitope; validate conditions in the relevant tissue type.
• In immunofluorescence/ICC, co-staining with a mitochondrial marker can help confirm specific localization.
• Cross-reactivity with mouse and rat orthologs is predicted; however, sequence alignment is advised to assess epitope conservation before use in these species.
• As a polyclonal antibody, lot-to-lot variability is possible; always titrate and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-COX15 polyclonal antibody is well-suited for studies of mitochondrial heme A biosynthesis and cytochrome c oxidase assembly, and we can also assist with sourcing rare-target reagents or custom antibody development to accelerate your projects from concept to clinic.