Anti-COX1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00397

Core Product Specifications and Parameters

Biological Background: Cytochrome c Oxidase Subunit 1 Function and Localization

• COX1, also referred to as cytochrome c oxidase subunit 1 or cytochrome c oxidase polypeptide I, is a core subunit of the mitochondrial cytochrome c oxidase (complex IV).
• It is an integral component of the respiratory chain, catalyzing the terminal reduction of molecular oxygen to water, a critical step in oxidative phosphorylation.
• The protein harbors the enzyme's active binuclear center, composed of heme A3 and copper B, where oxygen binding and reduction occur.
• Electrons from cytochrome c are transferred via a dinuclear copper A center in subunit 2 and heme A in subunit 1 to the binuclear center, facilitating proton translocation across the inner mitochondrial membrane.
• COX1 is embedded in the mitochondrial inner membrane, possessing multiple transmembrane helices that anchor it within the lipid bilayer.
• Key cofactors and metal-binding sites—including heme, copper, and magnesium—are essential for its electron transport and catalytic activities.
• As a vital component of the electron transport chain, COX1 contributes to the establishment of the mitochondrial membrane potential and ATP synthesis.

Experimental Guidance and Technical Tips

• For Western blotting, consider enriching mitochondrial fractions to improve detection sensitivity; the predicted molecular weight is approximately 57 kDa.
• In immunohistochemical or immunofluorescence applications, standard antigen retrieval methods may be employed, and validation in positive control tissues (e.g., heart, muscle) is recommended.
• Given the broad species cross-reactivity, the antibody can be applied across human, mouse, and rat experimental models, but individual validation is advised.
• For ELISA, pairing with a suitable capture or detection antibody may enhance assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined path to validated IVD raw materials, including this anti-COX1 monoclonal antibody. We provide comprehensive solutions spanning mitochondrial biology and respiratory chain research, such as optimized antibody pairs, bulk ancillary reagents, and custom sourcing for even the most elusive targets. Let us be your bridge from concept to clinic.