Anti-COX IV Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P19783

Core Product Specifications and Parameters

Biological Background: COX IV Function and Localization

• Cytochrome c oxidase subunit 4 isoform 1 (COX IV-1) is a nuclear-encoded component of mitochondrial complex IV, the terminal enzyme of the respiratory chain.
• It participates in the transfer of electrons from cytochrome c to molecular oxygen, a critical step that contributes to the proton gradient used for ATP synthesis.
• The protein is encoded by the Cox4i1 gene (also known as Cox4, Cox4a, Coxiv) and is part of a conserved family of cytochrome c oxidase subunits.
• COX IV-1 is an integral membrane protein located in the mitochondrial inner membrane, where it helps anchor the oxidase complex.
• The protein undergoes acetylation and phosphorylation, suggesting regulatory post-translational modifications that may influence enzyme activity or assembly.
• Structurally, COX IV-1 contains a transit peptide for mitochondrial targeting and transmembrane helices that ensure proper insertion into the membrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 23-98 of mouse COX IV, which may recognize both the precursor and mature forms of the protein. For Western blot, expect a band near 20 kDa; consider using mitochondrial fractionation or appropriate controls to confirm specificity.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to preserve mitochondrial morphology. Counterstaining with mitochondrial markers (e.g., MitoTracker) can help confirm localization.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; test citrate or EDTA-based protocols on your specific tissue type.
• The antibody cross-reacts with human, mouse, and rat COX IV; however, it is advisable to validate the reactivity in the relevant sample system before large-scale experiments.
• For ELISA applications, pairing with a capture antibody or testing multiple coating conditions can improve sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by connecting diagnostic manufacturers and research laboratories with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing solutions. With extensive supply networks, CamelBio supports mitochondrial research and oxidative phosphorylation studies by providing this COX IV antibody alongside complementary reagents, helping you accelerate from concept to clinic with reliable, scalable raw-material access.