Anti-COX IV Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - P13073

Core Product Specifications and Parameters

Biological Background: COX IV Function and Localization

• COX4I1 encodes cytochrome c oxidase subunit 4 isoform 1, a nuclear-encoded subunit of complex IV (cytochrome c oxidase) of the mitochondrial respiratory chain.
• This subunit is essential for the assembly and stability of the cytochrome c oxidase complex, which catalyzes the final step in the electron transport chain: the reduction of molecular oxygen to water.
• Electrons from cytochrome c are transferred via the dinuclear copper A center of subunit 2 and heme A of subunit 1 to the active binuclear center (heme A3 and copper B) in subunit 1, driving proton pumping across the inner membrane.
• The protein localizes to the mitochondrial inner membrane, featuring a transmembrane helix and a cleavable mitochondrial transit peptide.
• COX4I1 is ubiquitously expressed across tissues, reflecting the housekeeping role of cytochrome c oxidase in cellular respiration.
• Key post-translational modifications include acetylation and phosphorylation; mutations in COX4I1 are linked to primary mitochondrial disease (MC4DN16, mitochondrial complex IV deficiency, nuclear type 16).
• The protein is a target for proteomics identification and direct protein sequencing, highlighting its biomedical significance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 23–98 of human COX IV, a region conserved in mouse and rat, which aligns with the observed multi-species cross-reactivity; verify reactivity in your samples if non-rodent species are used.
• For western blotting, a 20 kDa band corresponding to mature COX IV is expected; consider using mitochondrial enrichment to enhance signal.
• The antibody is compatible with multiple immunoassays (WB, IP, IF/ICC, IHC-P, ELISA); optimize dilutions and protocols for each application, as performance may vary with sample preparation and detection systems.
• When performing immunohistochemistry or immunocytochemistry, mitochondrial localization patterns should be observed; use appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop supply of IVD raw materials, including validated monoclonal and polyclonal antibodies for mitochondrial targets like COX IV. Our portfolio extends to optimized antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing, helping you accelerate assay development from concept to clinic. For your oxidative phosphorylation and mitochondrial disease research needs, rely on CamelBio's integrated procurement solutions.