Anti-COX IV Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P13073

Core Product Specifications and Parameters

Biological Background: COX IV Function and Localization

• Cytochrome c oxidase subunit 4 isoform 1 (COX IV-1) is a nuclear-encoded subunit of complex IV (cytochrome c oxidase), the terminal enzyme of the mitochondrial electron transport chain.
• It plays a critical role in oxidative phosphorylation by contributing to the assembly and regulation of the holoenzyme, which catalyzes the reduction of molecular oxygen to water.
• The enzyme couples electron transfer from cytochrome c to oxygen with the translocation of protons across the inner mitochondrial membrane, generating the proton gradient essential for ATP synthesis.
• COX IV-1 is encoded by the COX4I1 gene and is ubiquitously expressed in human tissues, with a molecular weight of approximately 19.5 kDa for the mature protein.
• The protein is anchored in the mitochondrial inner membrane and is synthesized with a cleavable transit peptide that directs mitochondrial import.
• Post-translational modifications include acetylation and phosphorylation, which may modulate complex IV activity and assembly.
• Mutations in COX4I1 are linked to mitochondrial complex IV deficiency, nuclear type 16 (MC4DN16), a severe multisystem disorder.

Experimental Guidance and Technical Tips

• The immunogen used (N-terminal peptide within amino acids 1–100) suggests epitope recognition in a region that is conserved across human, mouse, and rat, supporting the observed cross-reactivity. Consider this when designing isoform-specific detection assays.
• For Western blotting, the predicted molecular weight of COX IV is ~20 kDa; validate detection using appropriate positive control lysates (e.g., mitochondrial fractions from HeLa, HEK293, or rodent liver).
• In immunohistochemistry (paraffin), standard antigen retrieval methods (citrate buffer, pH 6.0, or EDTA, pH 9.0) and endogenous peroxidase blocking should be applied; tissue-specific expression patterns may require optimization.
• When performing ELISA, the antibody may be used as a capture or detection reagent for native or recombinant COX IV; cross-reactivity with complex IV holocomplex or subassemblies may influence assay design—empirical validation is recommended.
• Always include species-matched controls and, if studying disease models, confirm the presence of mutations that might alter epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a dedicated one-stop sourcing partner for IVD manufacturers and research laboratories, offering seamless access to a broad portfolio of raw materials, technical services, and consultative support—from early-stage concept to clinical application. For targets like cytochrome c oxidase subunit IV, central to mitochondrial bioenergetics and disease biomarker studies, we provide validated antibody pairs, optimized recombinant monoclonal/polyclonal antibodies, ancillary reagents (buffers, detection systems), and bulk supply capabilities. Whether you need routine catalog products or assistance in sourcing rare and esoteric raw materials, CamelBio bridges your discovery pipeline with reliable, scalable solutions.