Anti-COUP-TFII/NR2F2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P24468

Core Product Specifications and Parameters

Biological Background: COUP-TFII/NR2F2 Function and Localization

• COUP-TFII (COUP transcription factor 2), also known as ARP-1 or NR2F2, is a member of the nuclear receptor subfamily 2 group F and functions as a ligand-activated transcription factor. It is activated by 9-cis-retinoic acid and all-trans-retinoic acid but not by dexamethasone, cortisol, or progesterone.
• The protein regulates apolipoprotein A-I gene transcription by binding to DNA site A. It may also play a critical role in establishing ovary identity during early gonad development. Related references: PMID:29478779
• COUP-TFII contains zinc-finger DNA-binding domains and belongs to the nuclear hormone receptor family, featuring both DNA-binding and ligand-binding domains.
• The protein localizes primarily to the nucleus, consistent with its role as a transcription regulator.
• It is ubiquitously expressed, with notable presence in the stromal cells of developing fetal ovaries.
• Multiple isoforms exist due to alternative splicing, and the protein undergoes phosphorylation, as identified by proteomic studies.
• Keywords associated with COUP-TFII include activator, DNA-binding, metal-binding, zinc-finger, receptor, transcription, and phosphoprotein, reflecting its functional versatility.

Experimental Guidance and Technical Tips

• The immunogen was designed against the N-terminal region (amino acids 5-100) of human COUP-TFII, which may ensure specificity for endogenous protein detection across multiple species.
• For Western blot (WB), consider using whole-cell lysates with standard protocols and validate with positive and negative controls. A band near 46 kDa is expected.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and antibody concentration to enhance signal-to-noise ratio.
• For immunoprecipitation (IP), this rabbit monoclonal antibody may provide high affinity; confirm complex formation with appropriate buffers and non-denaturing conditions.
• Given the cross-reactivity with mouse and rat, the antibody can be used for preclinical models; validate species-specific reactivity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to commercialization. This COUP-TFII/NR2F2 rabbit monoclonal antibody is part of our extensive portfolio for nuclear receptor and transcription factor research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and provide sourcing services for rare-target raw materials to accelerate your assay development.