Anti-COTL1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14019

Core Product Specifications and Parameters

Biological Background: Coactosin-Like Protein Function and Localization

• COTL1 (Coactosin-like protein, also known as CLP) is a member of the coactosin family of actin-binding proteins, with an apparent molecular weight of ~16 kDa.
• It binds to F-actin in a calcium-independent manner but does not directly promote actin depolymerization.
• COTL1 acts as a specific chaperone for 5-lipoxygenase (ALOX5/5LO), stabilizing the enzyme and thereby positively regulating leukotriene biosynthesis.
• The protein is predominantly cytoplasmic and associates with the cytoskeleton; it also localizes to the nucleus under certain conditions.
• Expression is widespread, with highest levels in placenta, lung, kidney, and peripheral blood leukocytes, and lower levels in brain, liver, and pancreas.
• Post-translational modifications include acetylation; the protein’s 3D structure has been solved, contributing to the understanding of its actin-binding and chaperone functions.
• Key functional annotations from UniProtKB include Actin-binding, Chaperone, and Cytoskeleton.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 142 amino acids of human COTL1, a region that includes the actin-binding domain and is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• In Western blot, a single band of approximately 16 kDa is expected; use low molecular weight markers and consider extended transfer times to prevent loss of small proteins.
• For immunohistochemistry (paraffin), antigen retrieval conditions may need optimization; known high-expression tissues such as placenta or leukocyte-rich tissues can serve as positive controls.
• The antibody can be used in ELISA to detect native or recombinant COTL1; validate coating and blocking conditions with your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering one-stop access to high-quality antibodies, antigens, and ancillary reagents for diagnostic and research applications. For COTL1-focused projects—including leukotriene signaling and cytoskeleton research—we supply validated antibody pairs, monoclonal/polyclonal antibodies, and bulk reagents. Our comprehensive sourcing capabilities also cover rare targets, enabling seamless progression from assay development to clinical application.