Anti-Cortactin Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14247

Core Product Specifications and Parameters

Biological Background: Cortactin Function and Localization

• Cortactin, encoded by the CTTN gene (also designated EMS1), is a Src substrate and actin-binding protein. Alternative splicing generates multiple isoforms, including the widely studied Amplaxin/EMS1 variants.
• Contributes to actin cytoskeleton organization, lamellipodia formation, and cell migration (PubMed:21296879). Related references: PMID:21296879
• In complex with ABL1 and MYLK, regulates cortical actin rearrangement essential for sphingosine 1-phosphate-mediated endothelial barrier enhancement (PubMed:20861316). Related references: PMID:20861316
• Plays a role in intracellular protein transport and endocytosis, modulating potassium channel surface levels (PubMed:17959782). Related references: PMID:17959782
• Required for stabilization of KCNH1 potassium channels at the plasma membrane (PubMed:23144454). Related references: PMID:23144454
• Promotes cancer cell invasiveness and metastasis formation, highlighting its significance in tumor progression (PubMed:16636290). Related references: PMID:16636290
• Localizes to diverse subcellular compartments, including the cytoplasm, cell cortex, lamellipodia, ruffles, podosomes, focal adhesions, clathrin-coated pits, dendritic spines, and endoplasmic reticulum.
• Subject to multiple post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination) and contains an SH3 domain, coiled-coil region, and cortactin repeats critical for protein–protein interactions.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 200 amino acids; consider validating detection of both full-length and N-terminal cortactin fragments in your sample system.
• For WB, use a standard 8–10% SDS-PAGE gel and short transfer times to resolve the ~62 kDa band; include positive controls (e.g., HeLa, A431, NIH/3T3 lysates) to confirm reactivity across human, mouse, and rat.
• For IHC-P, optimize antigen retrieval (e.g., citrate buffer pH 6.0) and anticipate both cytoplasmic and membranous staining patterns consistent with cortactin’s subcellular distribution.
• When using for ELISA, titration is recommended; the polyclonal format may allow direct detection, or it can be paired with a distinct epitope-specific detection antibody for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop supply chain for diagnostic manufacturers and researchers seeking IVD-grade antibodies against cytoskeletal and cancer targets. From validated cortactin antibodies to bulk ancillary reagents and customized sourcing of rare-target raw materials, our platform streamlines procurement across the entire development pipeline. Whether you need polyclonal antibodies, antigen-grade proteins, or technical consultation, CamelBio acts as your dedicated bridge from concept to clinic.