Anti-Cortactin Rabbit Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - Q14247

Core Product Specifications and Parameters

Biological Background: Cortactin Function and Localization

• Cortactin (also known as Amplaxin or EMS1) is encoded by the CTTN gene and acts as a substrate for Src kinase, contributing to the organization of the actin cytoskeleton and maintenance of cell shape. Related references: PMID:21296879
• It plays a key role in the formation of lamellipodia and promotes cell migration, essential for processes such as wound healing and tissue remodeling.
• Cortactin regulates neuronal morphology, axon growth, and dendritic spine density through interactions with CTTNBP2 and other partners.
• In complex with ABL1 and MYLK, it mediates cortical actin cytoskeletal rearrangement critical for sphingosine 1-phosphate (S1P)-dependent endothelial barrier enhancement. Related references: PMID:20861316
• It is involved in intracellular protein transport, endocytosis, and modulation of potassium channel (KCNH1) levels at the cell membrane, including receptor-mediated endocytosis via clathrin-coated pits. Related references: PMID:17959782 PMID:23144454
• Cortactin contributes to the invasiveness of cancer cells and the formation of metastases, highlighting its role in tumor progression. Related references: PMID:16636290
• It localizes to multiple subcellular sites including the cytoplasm, cytoskeleton, lamellipodia, ruffles, dendrites, cell membrane, podosomes, focal adhesions, and clathrin-coated pits.
• The protein undergoes various post‑translational modifications such as phosphorylation, acetylation, methylation, and ubiquitination, which regulate its function and interactions.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to the N‑terminal region (aa 1‑100) of human Cortactin, suggesting the antibody may recognize full‑length cortactin and possibly N‑terminal fragments.
• For Western blotting, a specific band around 62 kDa is expected in samples expressing cortactin; consider validating molecular weight markers.
• When using immunofluorescence (IF‑P) or immunohistochemistry (IHC‑P), optimize fixation, permeabilization, and antigen retrieval conditions for your specific tissue or cell type.
• This antibody has been tested on human, mouse, and rat samples; cross‑reactivity with other species should be verified experimentally if needed.
• For ELISA, pairing with a suitable detection antibody may be required; consider checkerboard titrations to determine optimal concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one‑stop platform for IVD raw materials, technical services, and consulting—from concept to clinic. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets like Cortactin, supporting studies on actin cytoskeleton dynamics and cancer biology. Contact us to streamline your procurement and accelerate your development pipeline.