Anti-Cortactin Polyclonal Antibody for WB, IF/ICC, ELISA - Q14247

Core Product Specifications and Parameters

Biological Background: Cortactin Function and Localization

• Cortactin (also known as Amplaxin or EMS1) is a 62 kDa actin-binding protein and Src kinase substrate that orchestrates actin cytoskeleton organization and cell shape maintenance. Related references: PMID:21296879
• It drives lamellipodium-based cell migration and participates in focal adhesion assembly and turnover.
• Cortactin regulates neuronal morphology, including axon growth, growth cone dynamics, and dendritic spine density through interactions with CTTNBP2.
• In endothelial cells, cortactin mediates S1P-induced cytoskeletal rearrangements essential for barrier function. Related references: PMID:20861316
• The protein is involved in clathrin-mediated endocytosis, intracellular transport, and stabilization of ion channels (e.g., KCNH1) at the cell membrane. Related references: PMID:17959782, PMID:23144454
• Pathologically, cortactin promotes cancer cell invasiveness and metastasis. Related references: PMID:16636290
• It dynamically localizes to the cytoplasm, cortex, lamellipodia, ruffles, podosomes, focal adhesions, dendritic spines, and clathrin-coated pits.
• Cortactin is subject to phosphorylation, acetylation, and ubiquitination, which fine‑tune its interactions and function.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band of ~62 kDa is expected. Validate the antibody using whole‑cell lysates from human, mouse, or rat cell lines known to express cortactin.
• For immunofluorescence (IF/ICC), consider using appropriate permeabilization to detect cortactin at membrane‑associated structures such as lamellipodia, ruffles, and focal adhesions.
• When performing ELISA, optimize coating and detection conditions using the recombinant immunogen fragment as a positive control.
• Because cortactin undergoes alternative splicing, additional bands may be observed; validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, offering validated polyclonal and monoclonal antibodies, including this anti‑cortactin antibody, along with bulk ordering and custom sourcing. Our portfolio supports cytoskeleton and oncology researchers with reliable reagents, from antibody pairs to ancillary proteins, streamlining your path from concept to clinic.