Anti-CORO1B Rabbit Polyclonal Antibody for WB and ELISA - Q9BR76

Core Product Specifications and Parameters

Biological Background: CORO1B Function and Localization

• CORO1B (Coronin-1B), also known as Coronin-2, is a member of the coronin family of actin-binding proteins characterized by multiple WD repeat domains.
• It plays a key role in regulating actin dynamics at the leading edge of migrating fibroblasts, thereby controlling cell motility.
• CORO1B is implicated in cytokinesis and signal transduction pathways that coordinate cytoskeletal rearrangements.
• The protein localizes to the cytoplasm, associates with the actin cytoskeleton, and is enriched in stress fibers.
• Post-translational phosphorylation of CORO1B likely modulates its activity and interactions within the actin cytoskeleton.
• Structural features include an N-terminal WD repeat domain, a conserved coiled-coil region, and a C-terminal domain that enable actin binding and protein-protein interactions.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 54 kDa. Validate specificity using appropriate positive and negative controls in your experimental system.
• The immunogen corresponds to the C-terminal region (aa 380-489) of human CORO1B; observed cross-reactivity with rat indicates epitope conservation across species.
• For ELISA, consider optimizing coating antigen and antibody dilutions; the recombinant immunogen can serve as a positive control when available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies for cytoskeleton and cell motility targets like CORO1B. Our one-stop sourcing model covers everything from polyclonal and monoclonal antibodies to bulk ancillary reagents and rare-target custom development, streamlining your path from concept to clinic.