Anti-CORO1A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P31146

Core Product Specifications and Parameters

Biological Background: Coronin-1A Function and Localization

• Coronin-1A (CORO1A), also known as p57, TACO, or CLIPINA, is an actin-binding protein of the coronin family characterized by WD repeats and a coiled-coil domain.
• It functions as a crucial component of the cytoskeleton in highly motile cells, facilitating the invagination of large plasma membrane regions and the formation of membrane protrusions during cell locomotion.
• In mycobacteria-infected cells, coronin-1A is retained on the phagosomal membrane, a process that prevents fusion between phagosomes and lysosomes, thereby contributing to intracellular pathogen survival.
• The protein is predominantly localized to the cytoplasm, cytoskeleton, cell cortex, and the membrane of cytoplasmic vesicles such as phagosomes.
• CORO1A is highly expressed in immune-related tissues including the thymus, spleen, bone marrow, and lymph node, with lower expression in lung and gut.
• Post-translational modifications include acetylation and phosphorylation, and the protein is known to undergo ubiquitination.
• Disease-associated variants of CORO1A are linked to immunodeficiencies, highlighting its critical role in immune cell function.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band of approximately 51 kDa is expected; consider using appropriate positive controls such as human thymus or spleen lysates.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval conditions should be optimized, and validation in known expressing tissues is advised.
• Although the antibody cross-reacts with mouse and rat, verify species-specific reactivity in your assay system.
• This polyclonal antibody is generated against a C-terminal fragment (aa 362-461); thus, it may recognize multiple isoforms if present.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of validated antibodies, including polyclonal and monoclonal options targeting cytoskeletal and immune-related proteins like coronin-1A. Our offerings extend to bulk ancillary reagents and custom rare-target sourcing, ensuring seamless integration from research to clinical assay development.