Anti-CORIN Polyclonal Antibody for WB, ELISA - Q9Y5Q5

Core Product Specifications and Parameters

Biological Background: CORIN Function and Localization

• CORIN (atrial natriuretic peptide-converting enzyme) is a serine-type endopeptidase belonging to the transmembrane protease serine family. It processes pro-atrial natriuretic peptide (pro-NPPA) and pro-brain natriuretic peptide (pro-NPPB) into their active hormones, ANP and BNP, which regulate blood pressure, natriuresis, diuresis, and vasodilation. Additionally, CORIN promotes trophoblast invasion and spiral artery remodeling in the pregnant uterus.
  Related references:
  PMID:10880574
  PMID:20489134
  PMID:21288900
  PMID:22437503
• Two isoforms exist: isoform 1 (canonical) exhibits full endopeptidase activity, while isoform 2 has significantly weaker catalytic function.
• Subcellularly, CORIN is primarily localized at the cell membrane as a single‑span transmembrane protein. It is also secreted into the bloodstream and can be detected in plasma and serum.
• Tissue expression is highest in the heart, specifically in cardiac myocytes. Significant expression is also observed in the pregnant uterus.
• Structural hallmarks include a signal‑anchor, a transmembrane helix, multiple disulfide bonds, N‑linked glycosylation, and a serine protease domain with autocatalytic activation from a zymogen form.
• Mutations in CORIN are associated with cardiomyopathy, underscoring its cardiovascular importance.
• Beyond cardiac function, CORIN may regulate sodium reabsorption in the kidney (by similarity).

Experimental Guidance and Technical Tips

• For Western blotting, consider using a reducing SDS‑PAGE protocol and a positive control such as human heart tissue or cardiac myocyte lysates. The predicted molecular weight is approximately 116 kDa; pre‑stained markers around this range are helpful.
• Since CORIN is a membrane‑associated protease, protein extraction protocols that enrich for membrane fractions may improve detection sensitivity.
• In ELISA applications, the recombinant immunogen (aa 1‑110) can serve as a coating antigen; validate assay conditions with known positive samples (e.g., recombinant CORIN or natural heart extracts).
• Cross‑reactivity with mouse and rat has been reported; however, confirm species‑specific reactivity in your own experimental setup using appropriate tissue controls.
• For studies involving soluble CORIN (shed form), consider using serum or plasma samples, but note that normal plasma levels may be low.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high‑quality IVD raw materials, offering everything from validated antibody pairs and optimized polyclonals to bulk ancillary reagents and rare‑target sourcing. For researchers focusing on cardiovascular biomarkers or natriuretic peptide pathways, this anti‑CORIN antibody exemplifies our commitment to supporting your entire development journey—from early‑stage research to scalable manufacturing. Contact us to discuss custom sourcing and technical consultation tailored to your project needs.