Anti-CoREST/RCOR1 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA, ChIP - Q9UKL0

Core Product Specifications and Parameters

Biological Background: CoREST/RCOR1 Function and Localization

• CoREST/RCOR1 is encoded by the RCOR1 gene and is also known as protein CoREST, functioning as a REST corepressor 1.
• It is an essential component of the BHC corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells by deacetylating and demethylating histones, acting as a chromatin modifier.
• In the BHC complex, CoREST serves as a molecular beacon for the recruitment of silencing machinery including MeCP2 and SUV39H1 to impose silencing across chromosomal intervals.
• Plays a central role in demethylation of Lys-4 of histone H3 by promoting the demethylase activity of KDM1A on core histones and nucleosomal substrates, and protects KDM1A from proteasomal degradation.
• Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via HDAC recruitment, genes implicated in multilineage blood cell development, controlling hematopoietic differentiation.
• Predominantly localized to the nucleus, consistent with its role in transcriptional regulation.
• Ubiquitously expressed across tissues.
• Annotated as a chromatin regulator, repressor, phosphoprotein, and involved in host-virus interaction among other keywords.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant protein spanning amino acids 256–485 of human CoREST (NP_055971.2). This region covers critical functional domains; consider verifying reactivity with your target isoform.
• WB application: A band corresponding to the predicted molecular weight of 53 kDa is expected; validate using positive control lysates from human or mouse origin.
• IF/ICC: Nuclear localization is anticipated; include appropriate permeabilization and blocking steps.
• IP and ChIP: The antibody may be suitable for immunoprecipitation and chromatin immunoprecipitation; empirical optimization of antibody concentration and crosslinking conditions is recommended.
• Cross-reactivity: Reported cross-reactivity with human and mouse; performance with other species should be verified experimentally.
• General note: As a polyclonal antibody, lot-to-lot consistency should be considered for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research institutions, providing one-stop access to IVD raw materials and technical support from concept to clinic. With this CoREST/RCOR1 antibody, researchers studying chromatin regulation and transcriptional repression can leverage our extensive portfolio of validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our dedicated sourcing team also assists in acquiring rare target raw materials to streamline your assay development pipelines.