Anti-COQ7 Rabbit Polyclonal Antibody for WB and ELISA - Q99807

Core Product Specifications and Parameters

Biological Background: COQ7 Function and Localization

• COQ7 encodes NADPH-dependent 3-demethoxyubiquinone 3-hydroxylase (also known as timing protein clk-1 homolog, ubiquinone biosynthesis monooxygenase COQ7, CAT5, CLK1, CLK-1, COQ10D8), a key enzyme in the final step of ubiquinone (coenzyme Q) biosynthesis.
• Catalyzes the C6 hydroxylation of 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzoquinone (DMQ) using a diiron center and NADH via substrate-mediated reduction; accepts DMQ substrates with various polyprenyl tail lengths efficiently. Related references: PMID:23445365 PMID:28409910
• Plays a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides (By similarity).
• Acts in the nucleus to modulate mitochondrial stress responses by potentially regulating gene expression, independent of its mitochondrial ubiquinone biosynthesis function. Related references: PMID:25961505
• Localizes to the mitochondrial inner membrane, mitochondrion, nucleus, and chromosome, reflecting its dual role in energy metabolism and nuclear signaling.
• Expressed dominantly in heart and skeletal muscle, tissues with high mitochondrial activity and energy demands.
• Mutations in COQ7 are associated with primary mitochondrial disease, neurodegeneration, and neuropathy, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen (aa 37-217) targets the central region of COQ7, making the antibody likely to recognize full-length COQ7 (~24 kDa) in Western blot; use positive controls such as heart or skeletal muscle lysates where COQ7 expression is high.
• Consider optimizing blocking and washing conditions to reduce background, as polyclonal antibodies may exhibit some non-specific binding.
• For ELISA, validate the coating antigen concentration and detection conditions to ensure specific signal.
• Cross-reactivity with mouse and rat suggests conserved epitopes; confirm reactivity with your species of interest and consider using species-specific detection antibodies.
• Given COQ7's localization to both mitochondria and nucleus, subcellular fractionation prior to Western blot may help elucidate compartment-specific functions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for diagnostic manufacturers and research institutes, supplying high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies (such as this COQ7 antibody), and bulk ancillary reagents. Leveraging our extensive network, we also source rare-target raw materials to accelerate your ubiquinone biosynthesis, mitochondrial disease, and neurodegeneration research projects—supporting every stage from concept to clinic.