Anti-COQ10B Rabbit Polyclonal Antibody for WB and ELISA - Q9H8M1

Core Product Specifications and Parameters

Biological Background: COQ10B Function and Localization

• COQ10B (Coenzyme Q-binding protein COQ10 homolog B, mitochondrial) is a 27 kDa protein encoded by the COQ10B gene (UniProt Q9H8M1), belonging to the COQ10 family of coenzyme Q-binding proteins.
• It is essential for coenzyme Q function in the mitochondrial respiratory chain, likely acting as a chaperone or facilitating the transport of Q6 to respiratory complex catalytic sites (By similarity).
• Subcellularly, COQ10B localizes to the mitochondrial inner membrane, consistent with its role in oxidative phosphorylation.
• The protein contains a transit peptide for mitochondrial targeting and undergoes alternative splicing, potentially generating isoforms with distinct regulatory properties.

Experimental Guidance and Technical Tips

• This polyclonal antibody is suitable for Western blot detection of endogenous COQ10B in human, mouse, and rat samples, given its reported cross-reactivity.
• For ELISA, the recombinant protein immunogen (amino acids 38–150) can serve as a robust positive control; consider pairing with this antibody for quantitative assays.
• When establishing new protocols, optimize antibody dilution, blocking reagents, and detection conditions in your specific sample matrix to ensure specificity and sensitivity.
• Note that polyclonal antibodies may exhibit lot-to-lot variability; always validate performance with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering a wide range of validated antibodies, optimized polyclonal and monoclonal reagents, and ancillary consumables tailored for mitochondrial biology and coenzyme Q research. From bulk supplies of this COQ10B antibody to customized antibody-pair development and rare-target raw-material procurement, we provide end-to-end support to accelerate your diagnostic and research workflows.