Anti-COPS7A Rabbit Polyclonal Antibody for WB, ELISA - Q9UBW8

Core Product Specifications and Parameters

Biological Background: COPS7A Function and Localization

• COPS7A (COP9 signalosome complex subunit 7a), also known as CSN7a or Dermal papilla-derived protein 10 (DERP10), is a core subunit of the COP9 signalosome (CSN) complex.
• The CSN complex is a master regulator of the ubiquitin–proteasome system, primarily acting by catalyzing the removal of the ubiquitin-like protein NEDD8 from cullin subunits of SCF-type E3 ubiquitin ligases (deneddylation), which inactivates these ligases.
• Beyond deneddylation, CSN associates with kinases such as casein kinase 2 (CK2) and protein kinase D (PKD) to phosphorylate a range of targets, including p53/TP53 (promoting its degradation), the transcription factor JUN (protecting it from degradation), IκBα, ITPK1, and IRF8, thereby linking signalosome function to multiple signaling pathways.
• Subcellular localization studies reveal that COPS7A distributes between the cytoplasm and the nucleus, consistent with its roles in both compartments.
• At the tissue level, COPS7A is widely expressed, with particularly high expression in brain, heart, and skeletal muscle.
• Post‑translational modifications observed on COPS7A include acetylation and phosphorylation; the protein also features a predicted coiled‑coil domain.
• UniProtKB annotates COPS7A with the keyword “Host‑virus interaction,” hinting at possible involvement in viral processes, although the mechanistic basis remains to be characterized.

Experimental Guidance and Technical Tips

• The immunogen spans the full‑length human COPS7A protein (amino acids 1–275), so the antibody is likely to detect both native and denatured forms. For Western blot, a major band near the predicted molecular weight of ~30 kDa is anticipated; slight shifts may occur due to post‑translational modifications.
• Cross‑reactivity with mouse and rat has been reported, suggesting conserved epitopes. Nonetheless, users should validate the antibody in their specific species and tissue context, using appropriate positive and negative controls.
• For ELISA, the antibody can be used as either a capture or detection reagent. Direct coating with recombinant COPS7A or with cell/tissue lysates may be optimized for sandwich or indirect ELISA formats.
• When performing Western blotting, consider including a loading control (e.g., GAPDH or β‑actin) and testing multiple tissue lysates (brain, heart, skeletal muscle) where COPS7A is highly expressed.
• Because COPS7A is part of a large multi‑subunit complex, mild lysis conditions and non‑denaturing gels may be helpful if studying CSN complex integrity, but denaturing SDS‑PAGE is appropriate for subunit detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the sourcing of IVD raw materials for diagnostic manufacturers, CROs, and research laboratories. This anti‑COPS7A polyclonal antibody is part of a growing portfolio of signalosome and ubiquitin‑pathway reagents. CamelBio can supply validated antibody pairs, bulk quantities, and ancillary reagents, and also assists with rare‑target raw‑material identification to accelerate your assay development from concept to clinic.