Anti-COPS2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P61201

Core Product Specifications and Parameters

Biological Background: COPS2 Function and Localization

• COPS2 (CSN2, TRIP15, Alien homolog) is an essential subunit of the COP9 signalosome (CSN) complex, a central regulator of the ubiquitin-proteasome system. The CSN complex mediates deneddylation of cullin-RING E3 ligases, thereby modulating their activity and controlling turnover of key signaling proteins.
• The COP9 signalosome also participates in kinase-mediated phosphorylation of substrates such as p53/TP53, c-Jun/JUN, and IkappaBalpha/NFKBIA, likely through its interaction with CK2 and PKD kinases, influencing their stability and downstream signaling.
• COPS2 localizes to both the cytoplasm and nucleus, consistent with its role in CRL regulation and transcriptional modulation.
• The protein is designated as a "signalosome" component and is classified under reference proteome, phosphoprotein, and alternative splicing in UniProt, indicating regulated expression and post-translational modification.
• COPS2 interacts with NIF3L1 to promote early neuronal differentiation, highlighting tissue-specific developmental functions.
• The UniProt record notes direct protein sequencing and proteomics identification as evidence for its existence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 310 amino acids; consider this when selecting detection antibodies or performing epitope mapping.
• For Western blot analysis, a specific band near 52 kDa should be observed; validate with appropriate positive controls (e.g., HeLa, HEK293 lysates).
• For IHC-P, optimize antigen retrieval conditions and use appropriate blocking steps to minimize nonspecific binding.
• The antibody cross-reacts with mouse and rat COPS2, enabling multi-species comparative studies.
• For IF/ICC, fixation and permeabilization methods may affect staining; test in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive, one-stop access to IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. This anti-COPS2 polyclonal antibody addresses the need for reliable reagents in ubiquitin-proteasome pathway studies and neuronal differentiation research. Beyond high-performance antibodies, we supply validated antibody pairs, bulk ancillary reagents, and can assist with rare target raw-material sourcing to accelerate your IVD project timelines.