Anti-COPG2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UBF2

Core Product Specifications and Parameters

Biological Background: COPG2 Function and Localization

• COPG2 encodes coatomer subunit gamma-2 (gamma-2-COP), a key component of the heptameric coatomer (COPI) complex.
• The coatomer complex mediates retrograde Golgi-to-ER transport of dilysine-tagged proteins, essential for maintaining organelle identity and protein homeostasis.
• Coatomer recruitment to Golgi membranes requires ADP-ribosylation factors (ARFs), small GTPases that regulate vesicle budding and cargo selection.
• Beyond retrograde trafficking, the COPI complex influences Golgi structural integrity, LDL receptor processing and recycling, and anterograde transport steps.
• COPG2 localizes to the cytoplasm, cytosol, Golgi apparatus membrane, and COPI-coated vesicles, dynamically cycling between these compartments.
• Alternative splicing of the COPG2 gene generates multiple isoforms, which may diversify coatomer function.
• The protein undergoes phosphorylation, a post-translational modification that may regulate coatomer assembly or membrane association.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 542–871 of human COPG2, covering the C-terminal region; consider aligning this sequence against mouse and rat orthologs to predict cross-reactivity characteristics.
• For Western blotting, anticipate a band near 98 kDa; use positive control lysates from HeLa, NIH/3T3, or RAT2 cells to confirm detection.
• For immunofluorescence or immunohistochemistry, expect Golgi-like perinuclear staining; co-stain with a Golgi marker (e.g., GM130) to validate localization.
• As a polyclonal antibody, lot-to-lot consistency should be verified; optimize working dilutions empirically for each application and model system.
• ELISA may be used for rapid screening of anti-COPG2 antibodies in coating or detection formats; include appropriate negative controls to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner supporting diagnostic manufacturers and research laboratories with a comprehensive portfolio of optimized antibodies, bulk reagents, and technical consultation. This anti-COPG2 polyclonal antibody serves researchers studying intracellular trafficking, Golgi biology, and coatomer-related diseases, and it complements our wider offering of validated antibody pairs, recombinant proteins, and auxiliary reagents. For inquiries about custom formulations, bulk supply, or support in transitioning this reagent into your assay pipeline, please contact our expert team.