Anti-COPG1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y678

Core Product Specifications and Parameters

Biological Background: COPG1 Function and Localization

• COPG1 encodes coatomer subunit gamma-1, a protein component of the COPI vesicle coat complex.
• The coatomer complex reversibly associates with Golgi non-clathrin-coated vesicles and binds dilysine motifs, mediating biosynthetic protein transport and retrograde Golgi-to-ER retrieval of escaped ER residents.
• Coatomer is required for budding from Golgi membranes, influencing Golgi structural integrity, LDL receptor processing and endocytic recycling.
• COPG1 participates in lipid homeostasis by limiting lipid droplet storage; it regulates perilipin family members PLIN2 and PLIN3 localization and promotes PNPLA2 (adipocyte triglyceride lipase) recruitment for lipolysis.
• Subcellular localization includes cytoplasm, Golgi apparatus membrane, and COPI-coated vesicle membrane.
• Post-translational modifications include phosphorylation; disease variants are annotated (UniProt keyword).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 555–874; the antibody likely detects full-length endogenous COPG1 at ~98 kDa in WB. Confirm specificity using positive and negative controls.
• For WB, consider using treated vs. untreated cell lysates (e.g., HeLa, NIH/3T3) to verify band size and upregulation under Golgi stress or lipolytic conditions.
• For IF/ICC, permeabilization is essential due to cytoplasmic and membrane-associated target localization; co-stain with a Golgi marker to validate.
• In ELISA, assess coating antigen format (peptide vs. recombinant protein) to ensure proper antibody capture.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-COPG1 polyclonal antibody supports cell biology and Golgi trafficking research. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to streamline your assay development.