Anti-COPE Rabbit Polyclonal Antibody for WB and ELISA - O14579

Core Product Specifications and Parameters

Biological Background: COPE Function and Localization

• COPE encodes the epsilon subunit of the coatomer complex, a cytosolic protein complex essential for COPI vesicle formation.
• The coatomer complex binds to dilysine motifs on cargo proteins and mediates retrograde transport from the Golgi to the ER, as well as intra-Golgi trafficking.
• COPE is involved in the structural integrity of the Golgi apparatus and influences processing, activity, and endocytic recycling of LDL receptors.
• The protein localizes to the cytoplasm, Golgi apparatus membrane, and COPI-coated vesicle membrane.
• Post-translational modifications include phosphorylation and ubiquitination (Ubl conjugation), suggesting regulatory mechanisms.
• Alternative splicing of COPE produces multiple isoforms, which may have distinct functions.
• The coatomer recruitment to membranes is dependent on ADP-ribosylation factors (ARFs), small GTP-binding proteins.

Experimental Guidance and Technical Tips

• The immunogen used spans the N-terminal 1-308 amino acids of human COPE; consider this region when verifying detection of specific isoforms or splice variants.
• This polyclonal antibody is suitable for Western blot (WB) and ELISA; optimal dilutions should be empirically determined for each application.
• Cross-reactivity is reported for human, mouse, and rat; validate in the relevant sample system if using other species.
• Due to the protein’s role in membrane trafficking, fractionation or enrichment protocols may enhance detection in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. For studies on COPI vesicle transport and Golgi biology, we provide validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our sourcing network also covers rare targets, ensuring reliable supply for your assay development needs.