Anti-COPB2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P35606

Core Product Specifications and Parameters

Biological Background: COPB2 Function and Localization

• COPB2 is the coatomer subunit beta', also known as beta'-COP or p102, and is encoded by the COPB2 gene.
• It is a core component of the coatomer complex, a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles.
• The coatomer complex is required for budding from Golgi membranes and mediates both anterograde biosynthetic transport from the ER to the trans-Golgi network and retrograde Golgi-to-ER transport of dilysine-tagged proteins.
• COPB2 is essential for the structural integrity of the Golgi apparatus and influences the processing, activity, and endocytic recycling of LDL receptors.
• The coatomer complex is recruited to Golgi membranes via GTP-dependent interaction with ADP-ribosylation factors (ARFs), and COPB2 can function as a selective binding protein (RACK) for protein kinase C epsilon type.
• Subcellularly, COPB2 localizes to the cytoplasm, cytosol, Golgi apparatus membrane, and COPI-coated vesicle membranes.
• The protein has a molecular weight of approximately 102 kDa and contains WD repeat domains, and it undergoes post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 657-906), so the antibody may recognize epitopes within this domain; consider this when designing epitope mapping or truncation experiments.
• For western blotting, expect a band near 102 kDa; validate specificity with appropriate controls, as COPB2 is ubiquitously expressed.
• In IHC-P, antigen retrieval conditions (e.g., citrate buffer pH 6.0) should be optimized; staining is expected in the cytoplasmic and perinuclear Golgi region.
• For IF/ICC, permeabilization with Triton X-100 or digitonin is recommended to access intracellular compartments; colocalization with Golgi markers can confirm specificity.
• The antibody has reported cross-reactivity with mouse and rat orthologs; however, sequence homology in the immunogen region should be verified for other species before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner, connecting research and diagnostic communities with high-quality reagents for vesicular trafficking and Golgi biology studies. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—all designed to streamline your assay development from concept to clinic. Our portfolio includes this COPB2 antibody and other tools to support your exploration of coatomer function and intracellular transport.