Anti-COPB2 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P35606

Core Product Specifications and Parameters

Biological Background: COPB2 Function and Localization

• COPB2 encodes the coatomer subunit beta'-COP (beta'-coat protein, p102), a core component of the COPI coatomer complex involved in intracellular vesicular trafficking.
• The coatomer complex binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, mediating biosynthetic protein transport from the ER through the Golgi to the trans Golgi network.
• COPB2 is essential for Golgi budding and retrograde Golgi-to-ER transport of dilysine-tagged proteins, maintaining Golgi structural integrity and regulating the processing, activity, and endocytic recycling of LDL receptors.
• COPB2 functions as a selective binding protein (RACK) for protein kinase C epsilon type, linking membrane trafficking with cell signaling through GTP-dependent binding to Golgi membranes.
• The protein localizes predominantly to the cytoplasm, Golgi apparatus membrane, and COPI-coated vesicle membranes, underscoring its role in COPI vesicle formation and cargo sorting.
• Post-translational modifications include acetylation and phosphorylation, which may modulate coatomer assembly, stability, or interactions with cargo and regulatory factors.
• Mutations in COPB2 are associated with primary microcephaly (MCPH19) and osteoporosis, highlighting its essential roles in cell division and bone homeostasis.
• The protein contains WD repeat domains, enabling protein-protein interactions critical for coatomer complex integrity and function.

Experimental Guidance and Technical Tips

• The immunogen (aa 657-906) spans the C-terminal WD repeat region; consider that the antibody may recognize conserved structural motifs when assessing cross-reactivity across species.
• For Western blot, the predicted molecular weight is 102 kDa; use an appropriate gel percentage (e.g., 8% SDS-PAGE) and optimize transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/ICC, expect a perinuclear, Golgi-like staining pattern; co-staining with a cis-Golgi marker (e.g., GM130) is recommended for validation.
• For ELISA, employ the recombinant immunogen as a positive control and standardize assay conditions with suitable blocking and wash buffers.
• Verify antibody specificity using COPB2 knockout or knockdown cell lysates if available, as COPB2 is essential and its depletion may affect cell viability.
• To preserve antibody activity, aliquot and store at -20°C or lower; avoid repeated freeze-thaw cycles and prolonged exposure to room temperature (shipped on ice bag).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-COPB2 rabbit monoclonal antibody supports research into Golgi trafficking, vesicular transport, and related disease mechanisms. Whether you require validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target sourcing, CamelBio streamlines your procurement with reliable supply and expert support.