Anti-COPA Polyclonal Antibody for WB and ELISA - P53621

Core Product Specifications and Parameters

Biological Background: COPA Function and Localization

• COPA encodes the alpha subunit of the coatomer complex (alpha-COP), a key component of COPI-coated vesicles involved in retrograde transport from the Golgi to the endoplasmic reticulum and intra-Golgi trafficking.
• The coatomer complex is a cytosolic hetero-oligomer that binds to dilysine motifs on cargo proteins and assembles onto Golgi membranes in an ADP-ribosylation factor (ARF)-dependent manner, facilitating vesicle budding.
• COPA is essential for maintaining Golgi structural integrity and plays roles in the processing, activity, and endocytic recycling of LDL receptors.
• An alternative splicing variant of COPA produces xenin, a peptide hormone found in gastrointestinal mucosa that stimulates pancreatic secretion and modulates gut motility, possibly via neurotensin receptors.
• COPA is uniformly expressed across a broad range of adult and fetal tissues; xenin is specifically detected in gastric, duodenal, and jejunal endocrine cells and circulates in blood.
• The protein localizes predominantly to the cytoplasm, Golgi apparatus membrane, and COPI-coated vesicle membranes, with the xenin fragment being secreted.
• Post-translational modifications include methylation and phosphorylation; mutations in COPA have been associated with disease variants (e.g., autoimmune interstitial lung, joint, and kidney disease).

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a synthetic peptide spanning amino acids 550–650 of human COPA; it is predicted to recognize the full-length protein (~138 kDa) in Western blot.
• For Western blot, consider using a suitable loading control and optimizing blocking conditions to reduce non-specific binding due to the polyclonal nature.
• Validate specificity in the appropriate cell or tissue lysate, such as HeLa cells or mouse tissues (given the reported mouse cross-reactivity).
• For ELISA, ensure that the coating antigen is compatible; the immunogen peptide or recombinant COPA protein can be used as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, supporting research and diagnostic development from early-stage biomarker discovery through clinical validation. For researchers investigating COPI vesicle transport and Golgi function, we offer not only this anti-COPA polyclonal antibody but also access to a wide range of validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate your assay development. Our sourcing capabilities extend to rare and custom targets, ensuring a reliable supply chain for your most demanding projects.