Anti-COPA Monoclonal Antibody for WB, IHC-P, ELISA - P53621

Core Product Specifications and Parameters

Biological Background: COPA Function and Localization

• COPA encodes coatomer subunit alpha (alpha-COP), a core component of the coatomer complex that coats COPI vesicles involved in protein trafficking between the endoplasmic reticulum and the Golgi apparatus.
• The coatomer complex binds to dilysine motifs on cargo proteins and mediates retrograde Golgi-to-ER transport, essential for maintaining Golgi structural integrity and function.
• COPA is a member of the WD repeat protein family, featuring multiple WD40 domains that facilitate protein-protein interactions within the coatomer complex.
• The protein is recruited to Golgi membranes by ADP-ribosylation factors (ARFs), small GTPases, and is involved in the endocytic recycling of LDL receptors.
• COPA is cleaved to produce the biologically active peptide xenin, which stimulates exocrine pancreatic secretion and modulates gastrointestinal motility via interaction with the neurotensin receptor.
• Subcellular localization includes cytoplasm, Golgi apparatus membrane, COPI-coated vesicle membrane, and secreted forms; it is uniformly expressed across a wide range of tissues, with xenin specifically found in gastric, duodenal, and jejunal mucosa.
• Post-translational modifications include phosphorylation and methylation, with alternative splicing and RNA editing generating isoforms that may be linked to disease variants.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (amino acids 1-100) of human COPA; this design may confer epitope specificity to this region. Consider this when interpreting results across species or isoforms.
• For Western blotting (WB), the predicted molecular weight is 138 kDa; use an appropriate gel percentage (e.g., 6-8% SDS-PAGE) and extended transfer times to ensure efficient membrane transfer of high-molecular-weight proteins.
• In immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized; COPA is expressed in cytoplasm and Golgi, so expect punctate perinuclear staining reflective of Golgi localization.
• For ELISA, the antibody may be used to capture or detect recombinant COPA or peptide fragments; validate with known standards and consider cross-reactivity with mouse and rat homologs.
• Cross reactivity data indicate reactivity with human, mouse, and rat; however, we recommend confirming reactivity in your specific experimental system before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials. This anti-COPA monoclonal antibody exemplifies our commitment to providing high-affinity, application-validated antibodies for intracellular trafficking and Golgi biology studies. Beyond single antibodies, we offer matched antibody pairs, bulk reagents, and custom sourcing for rare or difficult-to-procure targets, helping you streamline development from concept to clinic.