Anti-Contactin 2 Monoclonal Antibody for WB and ELISA - Q02246

Core Product Specifications and Parameters

Biological Background: Contactin-2 Function and Localization

• Contactin-2, also known as axonal glycoprotein TAG-1, axonin-1, and TAX-1, is a neural cell adhesion molecule encoded by the CNTN2 gene.
• It plays a critical role in the organization of axonal domains at the nodes of Ranvier by maintaining voltage-gated potassium channels at juxtaparanodal regions, in conjunction with CNTNAP2.
• Contactin-2 may also be involved in cell adhesion processes.
• The protein is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor.
• It belongs to the immunoglobulin superfamily and contains multiple immunoglobulin-like domains.
• UniProt keywords indicate potential associations with epilepsy, suggesting a role in neurological disorders.
• The protein is glycosylated and features a signal peptide for secretion/membrane targeting.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the N-terminal region (amino acids 1–100). Resulting antibodies are likely to recognize the extracellular domain of Contactin-2.
• For Western blot (WB), the predicted molecular weight is approximately 113 kDa. A band near this size is expected in human or mouse brain tissue lysates; optimization of antibody dilution may be required.
• For ELISA, the antibody can be used with immobilized peptide or recombinant Contactin-2 protein; consider testing a concentration range for optimal signal.
• Non-specific reactivity should be assessed in knockout or knockdown systems if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories comprehensive access to high-quality raw materials, technical services, and procurement support from concept to clinic. This Contactin-2 monoclonal antibody is ideal for neuroscience studies focusing on axon guidance and node of Ranvier architecture. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to meet your development needs.