Anti-Connexin 43 Polyclonal Antibody for WB, ELISA - P17302

Core Product Specifications and Parameters

Biological Background: Connexin 43 (GJA1) Function and Localization

• Gap junction alpha-1 protein, also known as Connexin-43 (Cx43), is encoded by the GJA1 gene and belongs to the connexin family.
• Structural component of gap junctions, forming channels that permit direct intercellular passage of ions and small molecules, enabling electrical and metabolic coupling.
• Negatively regulates bladder functional capacity by sensitizing bladder muscles to cholinergic stimuli through enhanced intercellular transmission.
• Plays roles in cardiac ventricular conduction and heart morphogenesis; may participate in potassium recycling in the cochlea essential for hearing.
• Functions in innate immune signaling by mediating the intercellular spread of antiviral second messengers such as 2',3'-cGAMP and 2-5A from infected cells to neighbors. Related references: PMID:24077100, PMID:31992625, PMID:40010341
• Subcellularly localizes to the cell membrane, gap junction plaques, and endoplasmic reticulum.
• Expressed at high levels in the heart (intercalated disks), fetal cochlea, and skin keratinocytes, with implications in cardiac and auditory physiology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal cytoplasmic domain (aa 283-382) of human Cx43, which is exposed and variable, favoring antibody accessibility and specificity. Consider this for detecting total Cx43 in Western blotting.
• In WB, the predicted molecular weight is ~43 kDa; validate band identity with relevant positive controls (e.g., heart or epithelial cell lysates) and consider phosphorylation-induced mobility shifts.
• For ELISA, optimize coating antigen and antibody dilution; note that cross-reactivity with mouse and rat allows multi-species sample testing.
• Verify antibody performance in your specific experimental system, as polyclonal antibodies may exhibit lot-to-lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like this anti-Connexin 43 pAb, and bulk ancillary reagents for gap junction biology and innate immunity research. For rare target sourcing or custom development needs, our integrated supply chain and technical team ensure reliable, end-to-end support from concept to clinic.