Anti-COMT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P21964

Core Product Specifications and Parameters

Biological Background: Catechol O-methyltransferase Function and Localization

• Catechol O-methyltransferase (COMT) catalyzes the O-methylation and inactivation of catecholamine neurotransmitters (e.g., dopamine, norepinephrine) and catechol hormones.
• It also methylates and shortens the biological half-lives of certain neuroactive drugs, such as L-DOPA, alpha-methyl DOPA, and isoproterenol.
• The enzyme is localized both in the cytoplasm and at the cell membrane, potentially existing as membrane-bound and soluble isoforms via alternative initiation.
• COMT is expressed in various tissues, including brain, liver, placenta, lymphocytes, and erythrocytes.
• It requires magnesium ions and S-adenosyl-L-methionine as a cofactor for its methyltransferase activity.
• COMT is implicated in neurotransmitter degradation and catecholamine metabolism, and has been associated with schizophrenia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 51–271 of human COMT, which includes the catalytic domain; this design may contribute to reactivity across human, mouse, and rat.
• For Western blotting, the predicted molecular weight is approximately 30 kDa; consider using a positive control such as brain or liver lysates where COMT is expressed.
• In immunofluorescence/ICC, COMT localizes to both cytoplasm and cell membrane; therefore, expect staining patterns reflecting these subcellular distributions. Validate in the relevant sample system.
• For ELISA applications, ensure proper coating and detection conditions are optimized with the target antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, bulk ancillary reagents, and custom procurement services for rare targets. For COMT research in neurotransmitter metabolism and related disorders, we provide reliable antibodies and technical support to bridge the gap from concept to clinic.