Anti-COMT Rabbit Polyclonal Antibody for WB, ELISA - P21964

Core Product Specifications and Parameters

Biological Background: COMT Function and Localization

• COMT (Catechol O-methyltransferase) catalyzes the O-methylation and inactivation of catecholamine neurotransmitters (dopamine, epinephrine, norepinephrine) and catechol hormones.
• It also methylates certain neuroactive drugs, such as L-DOPA, alpha-methyl DOPA, and isoproterenol, shortening their biological half-lives.
• The enzyme is localized to both the cytoplasm and the cell membrane, with alternative initiation yielding isoforms that may differ in subcellular distribution.
• COMT is expressed in brain, liver, placenta, lymphocytes, and erythrocytes, reflecting its broad role in catecholamine regulation.
• As a magnesium-dependent methyltransferase, COMT uses S-adenosyl-L-methionine (SAM) as the methyl donor.
• Post-translational modifications, including phosphorylation, can modulate its activity and stability.
• Key biological processes associated with COMT include catecholamine metabolism, neurotransmitter degradation, and lipid metabolism; it is also linked to schizophrenia in genetic studies.
• The predicted molecular weight of the canonical isoform is approximately 30 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 42-221 of human COMT (NP_009294.1). For Western blotting, a primary band at ~30 kDa is expected; confirm the exact molecular weight in your sample system and validate with appropriate controls.
• Due to potential alternative splicing and post-translational modifications, verify that the detected band(s) correspond to the target in your tissue or cell lysate.
• For ELISA, the recombinant immunogen can serve as a coating antigen; titrate the antibody and optimize blocking/detection conditions for best signal-to-noise ratio.
• The antibody is reported to cross-react with mouse and rat COMT; however, reactivity should be confirmed in the specific experimental model if used for non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibodies for metabolism and neuroscience targets like COMT. Whether you need polyclonal antibodies, matched antibody pairs, or ancillary reagents in bulk, our one-stop sourcing helps streamline your development pipeline. For specialized or difficult-to-source targets, we also offer tailored raw-material procurement solutions.