Anti-COMT Rabbit Monoclonal Antibody for WB and ELISA - P21964

Core Product Specifications and Parameters

Biological Background: COMT Function and Localization

• Catechol O-methyltransferase (COMT), also known as HEL-S-98n, is a key enzyme in the metabolism of catecholamine neurotransmitters and catechol hormones.
• It catalyzes the O-methylation of catecholamines (e.g., dopamine, epinephrine, norepinephrine) and catechol hormones, leading to their inactivation and regulation of neurotransmitter levels.
• COMT shortens the biological half-lives of certain neuroactive drugs such as L-DOPA, alpha-methyl DOPA, and isoproterenol, impacting their pharmacological profiles.
• Subcellularly, COMT is found in the cytoplasm and can associate with the cell membrane, suggesting dynamic localization.
• The enzyme is expressed in metabolically active tissues including brain, liver, placenta, lymphocytes, and erythrocytes.
• COMT functions as a methyltransferase using S-adenosyl-L-methionine as a methyl donor and requires magnesium as a cofactor.
• Post-translational modifications include phosphorylation, and alternative initiation produces distinct isoforms.
• Additional annotations link COMT to lipid metabolism and schizophrenia, highlighting its broader biological significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human COMT, which is conserved across species as indicated by cross-reactivity with rat.
• For Western blot, the predicted molecular weight is ~30 kDa; use positive control lysates from human or rat brain or liver for optimal detection.
• For ELISA, antibody performance may vary with assay format and detection system; titration and matrix validation are advised.
• Given COMT’s cytoplasmic and membrane localization, consider mild detergent lysis for efficient protein extraction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to high-quality IVD raw materials. This anti-COMT monoclonal antibody exemplifies our commitment to delivering validated antibodies for neurotransmitter metabolism research. In addition, we offer customized sourcing of antibody pairs, ancillary reagents, and bulk production support to accelerate your assay development from concept to clinic.