Anti-Complement Factor H Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08603

Core Product Specifications and Parameters

Biological Background: Complement Factor H Function and Localization

• Complement factor H (CFH) is a secreted glycoprotein that functions as a key soluble regulator of the complement alternative pathway, preventing excessive complement activation on host cells. Related references: PMID:21285368, PMID:21317894, PMID:25402769
• CFH accelerates the decay of the alternative pathway C3 convertase (C3bBb), thereby limiting the formation of C3b and downstream amplification of the complement cascade. Related references: PMID:19503104, PMID:21317894, PMID:26700768
• As a cofactor for serine protease factor I, CFH promotes the proteolytic degradation of deposited C3b, further terminating complement activity. Related references: PMID:18252712, PMID:23332154, PMID:28671664
• CFH mediates cellular responses by interacting with receptors such as CR3 (ITGAM), facilitating neutrophil adhesion to pathogens and subsequent phagocytosis. Related references: PMID:20008295, PMID:9558116
• The protein is primarily secreted, with high expression in the liver and additional local production by retinal pigment epithelium, monocytes, fibroblasts, and endothelial cells.
• CFH is associated with various diseases, including age-related macular degeneration (AMD) and atypical hemolytic uremic syndrome (aHUS), and features multiple sushi domains and disulfide bonds.
• Alternative splicing generates multiple isoforms (e.g., FHL1), providing additional functional diversity.
• In the mosquito midgut, CFH binds to the surface of P. falciparum gametocytes, protecting the parasite from alternative complement pathway-mediated elimination.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against an N-terminal region (aa 19-323) of human CFH. For Western blotting, consider the predicted molecular weight of approximately 139 kDa and validate in lysates from human, mouse, or rat sources.
• When using for immunohistochemistry (IHC-P), antigen retrieval protocols may enhance detection.
• For immunofluorescence (IF/ICC), appropriate fixation and permeabilization steps are recommended.
• ELISA application may require optimization of coating concentrations and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw-material procurement, offering a comprehensive portfolio including this high-quality anti-Complement Factor H polyclonal antibody. Beyond individual reagents, we supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your diagnostic development in complement biology and related disease areas.