Anti-Complement factor H Rabbit pAb for WB - P08603

Core Product Specifications and Parameters

Biological Background: Complement factor H Function and Localization

• Complement factor H (CFH; also known as H factor 1 or FHL1) is encoded by the CFH gene (HF, HF1, HF2) and functions as a soluble inhibitor of the complement alternative pathway.
• It binds self-surface glycans, preventing inappropriate complement activation and amplification on host cells.
  Related references: PMID:21285368, PMID:21317894, PMID:25402769
• CFH accelerates the decay of the alternative pathway C3 convertase (C3bBb) and acts as a cofactor for factor I to proteolytically degrade deposited C3b.
  Related references: PMID:19503104, PMID:21317894, PMID:26700768, PMID:18252712, PMID:23332154, PMID:28671664
• Via interaction with CR3/ITGAM on neutrophils, CFH mediates adhesion and phagocytosis of microbial pathogens.
  Related references: PMID:20008295, PMID:9558116
• CFH is a secreted protein (predicted MW 139 kDa), with the liver as its primary source; it is also expressed in retinal pigment epithelium, monocytes, fibroblasts, and endothelial cells.
  Related references: PMID:25136834, PMID:2139673, PMID:2968404, PMID:6444659
• Keywords and disease associations include complement pathway, innate immunity, age-related macular degeneration (AMD), and atypical hemolytic uremic syndrome (aHUS).

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 1–240 region, potentially recognizing full-length CFH and the truncated FHL1 isoform; verify specificity in systems where both may be present.
• For Western blotting, a band around 139 kDa is expected, though glycosylation and alternative splicing may yield additional bands; use appropriate positive controls.
• For immunofluorescence (IF/ICC or IF-P), optimize permeabilization to detect the secreted protein, and include a negative control for extracellular localization.
• In ELISA, consider pairing this polyclonal antibody with a monoclonal capture antibody for sandwich assays, and validate in the relevant species matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD manufacturers and research laboratories, providing validated antibodies, bulk ancillary reagents, and tailored technical services. This Complement factor H antibody supports studies of the complement alternative pathway, with potential applications in AMD and aHUS biomarker research. Whether you need antibody pairs, custom production, or regulatory guidance, CamelBio bridges your needs from concept to clinic.