Anti-Complement factor D Rabbit Polyclonal Antibody for WB, ELISA - P00746

Core Product Specifications and Parameters

Biological Background: Complement factor D Function and Localization

• Complement factor D (CFD), also designated Adipsin, C3 convertase activator, or Properdin factor D, is a serine protease that serves as the initiating enzyme of the alternative complement pathway, a critical component of innate immunity. Related references: PMID:21205667, PMID:22362762, PMID:6769474
• It functions by cleaving complement factor B (CFB) when CFB is bound to C3b, thereby forming the active C3 convertase (C3bBb) of the alternative pathway. Related references: PMID:21205667, PMID:6769474, PMID:874324
• The alternative pathway is initiated by spontaneous hydrolysis of complement C3, and CFD-mediated activation generates C3 and C5 convertases, which amplify the complement cascade and promote opsonization, inflammation, and lysis of pathogens. Related references: PMID:21205667, PMID:22362762, PMID:6769474
• CFD is a secreted protein, released into the bloodstream and extracellular fluids where it carries out its proteolytic function.
• It is synthesized as a preproprotein with a signal peptide and an activation peptide; the mature active enzyme is generated by proteolytic removal of the activation peptide. Structurally, it possesses canonical disulfide bonds characteristic of trypsin-like serine proteases.
• Mutations in the CFD gene are linked to complement deficiencies and increased susceptibility to infection, underscoring its essential role in immune defense.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein encompassing amino acids 26–253 of human CFD, which corresponds to the mature, active enzyme. Therefore, the antibody is expected to recognize both the pro-form and the active protease.
• In Western blot (WB) analysis, a specific band at approximately 27 kDa is anticipated under reducing conditions. Non-reducing conditions may reveal disulfide-linked multimers or conformational variants; consider testing both conditions.
• For ELISA applications, this polyclonal antibody can be used as a capture or detection reagent for CFD in biological fluids such as serum or plasma. Optimal antibody pairings and concentrations should be determined empirically.
• Due to the secreted nature of CFD, sample preparation may require attention to prevent degradation; adding protease inhibitors and processing samples at low temperatures is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for sourcing IVD raw materials, supporting diagnostic manufacturers at every stage from concept to clinic. For research on the complement system and inflammatory disorders, this anti-CFD antibody complements our portfolio of high-quality antibodies, validated antibody pairs, and bulk ancillary reagents. Whether you need ready-to-use reagents or assistance with rare target sourcing, CamelBio is your reliable partner for streamlined procurement and technical consultation.