Anti-Complement factor D Rabbit Monoclonal Antibody for WB, ELISA - P00746

Core Product Specifications and Parameters

Biological Background: Complement factor D Function and Localization

• Complement factor D (CFD; aliases: Adipsin, Properdin factor D, DF, ADN, PFD) is a secreted serine protease of the peptidase S1 family. It circulates as a zymogen and is activated by N-terminal propeptide cleavage.
• As the rate-limiting enzyme of the alternative complement pathway, CFD specifically cleaves factor B (CFB) when bound to C3b, forming the C3 convertase C3bBb.
  Related references: PMID:21205667, PMID:6769474, PMID:874324
• The alternative pathway is initiated by spontaneous C3 hydrolysis and functions as an amplification loop for all complement pathways, contributing to innate immune defense.
  Related references: PMID:21205667, PMID:22362762, PMID:6769474
• CFD is predominantly synthesized by adipocytes and secreted into the blood, where it acts extracellularly. Its subcellular location is therefore secreted/extracellular.
• The mature protein (~27 kDa) contains structurally conserved disulfide bonds characteristic of serine proteases.
• Keywords: complement alternate pathway, innate immunity, serine protease, hydrolase, secreted, disulfide bond, zymogen.

Experimental Guidance and Technical Tips

• The immunogen used is recombinant human CFD protein; therefore, the antibody is likely to recognize both native and denatured conformations of the target.
• For Western blot, the predicted molecular weight is 27 kDa. Under reducing SDS-PAGE, the protein may appear as a monomeric band around this size. Validate with human serum or plasma samples.
• For ELISA, consider using this antibody as a capture or detection reagent. Optimal working dilutions and pairing with other reagents must be determined experimentally in the intended assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive source for IVD raw materials, offering complement factor D antibodies, recombinant proteins, and ancillary reagents to support immunoassay development. Our portfolio includes validated monoclonal antibodies, bulk reagent supplies, and sourcing capabilities for rare targets in the complement pathway to simplify your procurement workflow.