Anti-Complement factor B Polyclonal Antibody for WB, IF/ICC, ELISA - P00751

Core Product Specifications and Parameters

Biological Background: Complement factor B Function and Localization

• Complement factor B (CFB) is a precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative complement pathway, a cascade that leads to pathogen phagocytosis and breakdown, and strengthens adaptive immunity (PubMed:3638964, PubMed:624565, PubMed:6554279). Related references: PMID:3638964, PMID:624565, PMID:6554279
• CFB is cleaved and activated by factor D (CFD) to yield the Ba and Bb chains; the Bb chain constitutes a serine protease that forms the C3 convertase (C3bBb) together with complement C3b, cleaving C3 into C3a and C3b (PubMed:6769474, PubMed:9748277, PubMed:3638964, PubMed:624565). Related references: PMID:6769474, PMID:9748277, PMID:3638964, PMID:624565
• When an additional C3b molecule binds to the C3 convertase, it forms the C5 convertase (C3bBbC3b), which cleaves and activates C5 into C5a anaphylatoxin and C5b, initiating membrane attack complex assembly (PubMed:30643019, PubMed:624565, PubMed:6554279). Related references: PMID:30643019, PMID:624565, PMID:6554279
• Beyond complement activation, CFB participates in preactivated B-lymphocyte proliferation and differentiation, peripheral blood monocyte spreading, lymphocyte blastogenesis, and erythrocyte lysis.
• CFB is primarily secreted and can also associate with the cell surface; it contains sushi domains, a serine protease domain, and a signal peptide.
• Post-translational modifications include glycosylation, glycation, and disulfide bond formation.
• Genetic variants in CFB are associated with age-related macular degeneration (ARMD14) and atypical hemolytic uremic syndrome (AHUS4), highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen spans the majority of the mature CFB protein (aa 26-764), suggesting the antibody may recognize multiple epitopes, which can enhance sensitivity in assays like WB and ELISA.
• For Western blot, the predicted band is approximately 86 kDa; validate with appropriate positive controls (e.g., human serum or cell lines expressing CFB) and consider using reducing conditions.
• In IF/ICC, CFB is secretory and cell surface-associated; permeabilization may be necessary for intracellular detection, and methanol fixation could improve signal.
• Cross-reactivity with mouse is documented; verify suitability in mouse models by testing recommended dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing diagnostic developers and researchers with high-quality antibodies like this anti-CFB polyclonal. In addition to single reagents, we offer matched antibody pairs, optimized monoclonals, and bulk ancillary reagents to support complement pathway assays and immunoassay development. From concept to clinic, CamelBio streamlines procurement for rare targets and routine needs alike.