Anti-Complement Factor B (CFB) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00751

Core Product Specifications and Parameters

Biological Background: Complement factor B Function and Localization

• Complement factor B (CFB) serves as the precursor of the catalytic component of the C3 and C5 convertase complexes in the alternative complement pathway, playing a central role in the amplification loop that enhances classical, lectin, and GZMK-driven complement activation. Related references: PMID:3638964, PMID:624565, PMID:6554279, PMID:6769474, PMID:9748277
• Upon cleavage by factor D (CFD), CFB generates Ba and Bb chains; the Bb chain is a serine protease that associates with complement C3b to form the C3 convertase (C3bBb), which then recruits an additional C3b molecule to become the C5 convertase. Related references: PMID:30643019, PMID:3638964, PMID:624565, PMID:6554279
• Beyond convertase activity, CFB is involved in B‑lymphocyte proliferation and differentiation, peripheral blood monocyte spreading, lymphocyte blastogenesis, and erythrocyte lysis, linking innate and adaptive immunity.
• CFB is a secreted glycoprotein also detected on the cell surface, consistent with its local complement activation roles.
• Post‑translational modifications include glycation and disulfide bond formation; the protein contains sushi repeat domains and is classified as a serine protease zymogen.
• Mutations in CFB are associated with age‑related macular degeneration and atypical hemolytic uremic syndrome, underscoring its clinical relevance.
• The mature protein has a calculated molecular weight of approximately 85.5 kDa, migrating around 86 kDa on SDS‑PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 26–259 of human CFB (NP_001701.2). Consider using the full‑length protein or the specific domain as a positive control.
• Suitable for Western blot (predicted band ~86 kDa), IHC‑P, IF/ICC, and ELISA. Optimize antibody dilution and blocking conditions for each application and sample type.
• Cross‑reactivity with mouse and rat CFB enables multispecies studies, but sequence homology should be verified when extending to other organisms.
• As a rabbit polyclonal, expect batch‑to‑batch consistency in specificity; always validate the antibody in your own experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti‑CFB antibody supports complement pathway research and diagnostic development. Explore our portfolio for validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw‑material sourcing tailored to your immunodiagnostic needs.