Anti-Complement factor B (CFB) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P00751

Core Product Specifications and Parameters

Biological Background: Complement factor B (CFB) Function and Localization

• CFB is a crucial component of the alternative complement pathway, serving as the precursor to the serine protease that forms the C3 and C5 convertases. Related references: PMID:3638964, PMID:624565, PMID:6554279
• Upon cleavage by factor D (CFD), CFB generates the catalytic Bb fragment, which complexes with C3b to form the C3 convertase C3bBb, and subsequently the C5 convertase upon binding another C3b. Related references: PMID:30643019, PMID:624565, PMID:6554279
• Activated Bb cleaves C3 and C5, producing anaphylatoxins C3a and C5a, and opsonin C3b; this amplifies complement activation and links to innate and adaptive immunity.
• CFB is also involved in B-lymphocyte proliferation and differentiation, monocyte spreading, and lymphocyte blastogenesis, highlighting its immunomodulatory roles beyond the complement cascade.
• The protein contains sushi (CCP) domains, a serine protease domain, and is a glycoprotein with disulfide bonds; it is secreted and can be found on cell surfaces.
• Clinically, mutations in CFB are associated with age-related macular degeneration (ARMD14) and atypical hemolytic uremic syndrome (AHUS4), indicating its importance in ocular and renal immune homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 400-500 (NP_001701.2), which lies within the Bb fragment (catalytic domain). This antibody may detect both the full-length proenzyme and the activated Bb fragment, making it suitable for monitoring CFB activation.
• For Western blot (WB), a band around 86 kDa is expected for full-length CFB in human and mouse samples; validate in serum or complement-stimulated cell models.
• For immunofluorescence/ICC, consider fixation methods that preserve secreted and cell-surface proteins; co-localization with complement components may require careful optimization.
• In ELISA applications, use appropriate positive controls (e.g., recombinant CFB or activated complement serum) and include proper negative controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials and technical services, from concept to clinic. This high-quality CFB monoclonal antibody supports complement pathway research and the development of IVD assays. CamelBio’s portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, ensuring reliable sourcing for complement-related and other rare-target projects.