Anti-Complement C2 Rabbit Polyclonal Antibody for WB and ELISA - P06681

Core Product Specifications and Parameters

Biological Background: Complement C2 Function and Localization

• C2 is the precursor of the catalytic component of the C3 and C5 convertase complexes, essential in the classical, lectin, and GZMK complement systems (PubMed:12878586, PubMed:17027507, PubMed:18204047). Related references: PMID:12878586 PMID:17027507 PMID:18204047
• Following complement activation, C2 is recruited to pathogen surfaces by C4b to form the C3 convertase, or by C3b and C4b to form the C5 convertase (PubMed:6611150, PubMed:6906228). Related references: PMID:6611150 PMID:6906228
• In the C3 convertase, C2 cleaves C3 into C3a anaphylatoxin and C3b opsonin; in the C5 convertase, it cleaves C5 into C5a and C5b, leading to membrane attack complex formation (PubMed:14561755, PubMed:17027507, PubMed:12878586, PubMed:18204047). Related references: PMID:14561755 PMID:17027507 PMID:12878586 PMID:18204047
• C2 is a secreted protein that also localizes to the cell surface.
• The protein contains multiple structural features and modifications, including disulfide bonds, glycosylation, and a serine protease domain.
• Genetic variants in C2 are associated with age-related macular degeneration and other complement-related disorders.
• Alternative splicing generates multiple isoforms of C2.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 453–752 of human C2; verify coverage of your region of interest if targeting specific isoforms or fragments.
• For Western blot, expect a band at approximately 83 kDa; include appropriate positive and negative controls from human serum or cell lysates.
• For ELISA, optimize coating antigen concentration and antibody dilutions; cross‑validation with a secondary detection system is recommended.
• As a polyclonal antibody, evaluate lot-to-lot consistency and specificity in your assay, potentially using purified C2 protein or complement‑depleted sera.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of IVD raw materials by offering a broad portfolio of validated antibodies, recombinant proteins, and ancillary reagents. For complement research and diagnostic development, we can source bulk quantities of C2 antibodies, matched pairs, and optimized monoclonals. Our integrated supply chain helps accelerate your projects from concept to clinic with reliable, traceable reagents.