Anti-COMP Polyclonal Antibody for WB, IF-P, ELISA - P49747

Core Product Specifications and Parameters

Biological Background: COMP (Cartilage Oligomeric Matrix Protein) Function and Localization

• COMP, also known as Thrombospondin-5 (TSP5), is a member of the thrombospondin family of extracellular matrix proteins.
• It plays a critical role in maintaining cartilage structural integrity by interacting with collagens, fibronectin, and cell surface integrin receptors (PubMed:16051604, PubMed:16542502). Related references: PMID:16051604, PMID:16542502
• Acts as a potent suppressor of apoptosis in chondrocytes by blocking caspase-3 activation and inducing IAP survival proteins (BIRC2/3/5, XIAP) (PubMed:17993464). Related references: PMID:17993464
• Essential for maintaining vascular smooth muscle cell (VSMC) contractile phenotype via interaction with ITGA7 (By similarity).
• Localized to the extracellular matrix and secreted into the extracellular space.
• Abundantly expressed in cartilage (especially in osteoarthritis), as well as in bone, tendon, ligament, synovium, and blood vessels.
• Contains EGF-like domains and calcium-binding repeats; undergoes alternative splicing and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 20-160) of human COMP; the antibody may recognize full-length COMP and certain splice isoforms.
• For Western blot, the predicted molecular weight is approximately 83 kDa (calculated 82.8 kDa). Consider using reducing conditions and appropriate controls.
• In immunofluorescence on paraffin-embedded (IF-P) tissues, antigen retrieval and permeabilization steps may be required for optimal detection.
• Cross-reactivity with mouse and rat has been documented; validation in species-specific sample systems is recommended.
• This polyclonal antibody is suitable for ELISA, but optimal working dilutions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic development. This anti-COMP antibody targets a key extracellular matrix protein implicated in cartilage biology and osteoarthritis, aligning with our portfolio for musculoskeletal disease research. Our team can assist with custom procurement and rare-target sourcing to streamline your assay development pipeline.